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Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics MSPP
Published on: October 30, 2016
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Mass Spectrometry-based PhyloProteomics (MSPP): A novel microbial typing Method
Andreas Erich Zautner1, Wycliffe Omurwa Masanta1, Michael Weig1
1Institut für Medizinische Mikrobiologie, Universitätsmedizin Göttingen, Kreuzbergring 57, 37075 Göttingen, Germany.
Scientific Reports
|August 26, 2015
Summary
Mass spectrometry-based phyloproteomics (MSPP) offers a novel microbial typing method using protein mass variations. This technique shows potential comparable to sequencing for identifying virulence and resistance factors.
Area of Science:
- Microbiology
- Proteomics
- Bioinformatics
Background:
- Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) is becoming a standard for microbial identification in clinical labs.
- Allelic variations in microbial proteins cause mass differences, enabling new typing strategies.
Purpose of the Study:
- To introduce and demonstrate mass spectrometry-based phyloproteomics (MSPP) as a novel microbial typing method.
- To compare the efficacy of MSPP with traditional multilocus sequence typing (MLST).
Main Methods:
- MSPP utilizes amino acid sequence variations from allelic protein isoforms detected as mass shifts in MALDI-TOF spectra.
- Hierarchical cluster analysis was employed to construct phyloproteomic dendrograms.
- The method was applied to a collection of Campylobacter jejuni isolates.
Main Results:
- MSPP successfully deduced amino acid sequences from biomarker masses for each isolate.
- Phylogenetic analysis using MSPP yielded results comparable to laborious sequencing-based typing methods like MLST and whole genome sequencing (wgMLST).
Conclusions:
- MSPP is a viable and efficient method for microbial typing, offering insights into evolutionary relatedness.
- The technique holds significant potential for identifying virulence and antimicrobial resistance factors linked to microbial evolution.
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