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Anionic Polymerization of an Amphiphilic Copolymer for Preparation of Block Copolymer Micelles Stabilized by π-π Stacking Interactions
Published on: October 10, 2016
[Study of a high molecular copolymer HFMC in vaginal irrigation for contraception in mice]
Abstract:
A vaginal spermicide of high molecular copolymer, ethyl methacrylate-methacrylic acid-hydroxyethyl methacrylate (HFMC), was introduced through a small tube into the mouse's vagina and made to adhere to it's wall, causing a pH alteration in the vaginal environment. Matching experiments showed that the sperms were killed or/and lost their mobility and vitality owing to the infertile acidic environment. The fertility could be restored when the HFMC dissolved gradually. But the delivering time was delayed for about 112-118 days versus the control (P less than 0.001). The fertility could also be restored artificially by flushing out the copolymer with solvent DMSO. In this case the delivering time was consistent with the control (P greater than 0.05).
Insights
A novel vaginal spermicide, HFMC, creates an acidic environment to reduce sperm motility. Fertility is restored upon copolymer dissolution or removal, with delayed delivery times observed.
Area of Science:
- Reproductive biology
- Biomaterials science
Context:
- Development of novel contraceptive methods.
- Investigating the impact of vaginal pH on sperm viability.
Purpose:
- To evaluate the efficacy of a high molecular copolymer, ethyl methacrylate-methacrylic acid-hydroxyethyl methacrylate (HFMC), as a vaginal spermicide.
- To assess the reversibility of HFMC's spermicidal effects and its impact on delivery time in a mouse model.
Summary:
- A copolymer (HFMC) was introduced into the mouse vagina, altering the pH and rendering sperm infertile by reducing motility and vitality.
- Fertility was restored upon gradual dissolution of HFMC, though delivery time was significantly delayed (112-118 days).
- Artificial restoration of fertility by flushing with DMSO resulted in delivery times consistent with controls, indicating reversibility.
Impact:
- Demonstrates the potential of HFMC as a reversible vaginal spermicide.
- Highlights the importance of vaginal pH modulation for contraceptive efficacy.
- Provides insights into the duration of contraceptive effect and methods for its reversal.
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