Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chromatin Packaging01:32

Chromatin Packaging

20.3K
Each human somatic cell contains 6 billion base pairs of DNA. Each base pair is 0.34 nm long, meaning each diploid cell contains a staggering 2 meters of DNA. This long DNA strand is packed inside a nucleus measuring only 10-20 microns in diameter with the help of specialized DNA-binding proteins called histones. Together they form a compact DNA-protein complex called chromatin. The chromatin is further compacted into higher-order structures. The highest level of compaction is achieved during...
20.3K
Chromatin Packaging02:21

Chromatin Packaging

23.3K
Each human somatic cell contains 6 billion base-pairs of DNA. Each base-pair is 0.34 nm long, which means that each diploid cell contains a staggering 2 meters of DNA. How is such a long DNA strand packed inside a nucleus measuring only 10 - 20 microns in diameter? 
The chromatin
In combination with specialized DNA binding protein called Histones, the DNA double helix forms a compact DNA: protein complex called chromatin. The chromatin itself is further compacted into higher-order...
23.3K
Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

2.3K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
2.3K
The Nucleosome01:19

The Nucleosome

4.8K
Human DNA is almost two meters long. However, it is compressed inside a tiny nucleus measuring only a few microns in diameter. To make this degree of compaction possible, DNA is organized into several sequential levels so that it can fit into such a tiny space. The most compact form of DNA is a chromosome that can be seen under a microscope in a dividing cell.
In a chromosome, DNA is wound twice around a protein complex called a histone octamer core, which consists of 8 histone proteins. This...
4.8K
The Nucleosome02:33

The Nucleosome

5.6K
5.6K
The Nucleosome02:33

The Nucleosome

19.8K
DNA in a human cell is almost 2m long and it is packed inside a tiny nucleus that is only a few microns in diameter. The level of compaction of DNA inside the nucleus is astonishing. It is organized into several sequentially higher levels of compaction to fit into such a tiny space. The most compact form of DNA is a chromosome that can be seen under a microscope in a dividing cell.
DNA is wound twice around a protein complex called histone core, that consist of 8 histone proteins. This complex...
19.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Author Correction: DNA polymerase epsilon is required for heterochromatin maintenance in Arabidopsis.

Genome biology·2026
Same author

Clinical features and outcomes of MPN with concurrent MPL and JAK2/CALR Mutations.

Blood neoplasia·2026
Same author

GLP-1 receptor agonist use and overall survival among women with type 2 diabetes and breast cancer: a retrospective cohort study.

The oncologist·2026
Same author

Identification of neoadjuvant chemoradiotherapy resistance-associated proteins in locally advanced rectal cancer: A pilot study.

Oncology letters·2026
Same author

Corrigendum to "Guipi Tang alleviates vascular dementia by regulating purine metabolism via gut microbiota-derived pantothenic acid" [Phytomedicine volume 148 (2025) 157297].

Phytomedicine : international journal of phytotherapy and phytopharmacology·2026
Same author

Selphi, a tool for improving genotype imputation accuracy.

Scientific reports·2026

Related Experiment Video

Updated: Apr 5, 2026

Chromatin Immunoprecipitation from Human Embryonic Stem Cells
10:36

Chromatin Immunoprecipitation from Human Embryonic Stem Cells

Published on: July 22, 2008

21.5K

Nucleosome Organization in Human Embryonic Stem Cells.

Puya G Yazdi1, Brian A Pedersen1, Jared F Taylor1

  • 1UC Irvine Diabetes Center, University of California Irvine, Irvine, California, United States of America; Sue and Bill Gross Stem Cell Research Center, University of California Irvine, Irvine, California, United States of America; Department of Medicine, University of California Irvine, Irvine, California, United States of America.

Plos One
|August 26, 2015
PubMed
Summary

DNA sequence dictates basic nucleosome organization in stem cells. Other factors like transcription and epigenetic marks modify this ground state, revealing insights into cellular identity and pluripotency circuitry.

More Related Videos

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C
09:32

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C

Published on: October 14, 2022

4.8K
CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells
11:13

CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells

Published on: May 25, 2018

10.3K

Related Experiment Videos

Last Updated: Apr 5, 2026

Chromatin Immunoprecipitation from Human Embryonic Stem Cells
10:36

Chromatin Immunoprecipitation from Human Embryonic Stem Cells

Published on: July 22, 2008

21.5K
Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C
09:32

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C

Published on: October 14, 2022

4.8K
CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells
11:13

CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells

Published on: May 25, 2018

10.3K

Area of Science:

  • Genomics
  • Epigenetics
  • Stem Cell Biology

Background:

  • Nucleosomes are fundamental to eukaryotic DNA packaging, transcriptional regulation, and cellular identity.
  • The determinants and significance of genome-wide nucleosome organization, especially in stem cells, remain largely unknown.

Purpose of the Study:

  • To investigate the primary drivers of nucleosome organization in human embryonic stem cells.
  • To understand the interplay between DNA sequence, transcription, and epigenetic modifications in shaping chromatin structure.

Main Methods:

  • Ultra-deep sequencing of nucleosomal DNA from human embryonic stem cell lines.
  • Integration of sequencing data with multiple epigenomic maps (e.g., histone modifications, DNA methylation).
  • Computational analysis of nucleosome organization patterns.

Main Results:

  • DNA sequence is a key determinant of nucleosome organization, establishing a 'ground state' in transcriptionally inactive regions.
  • Transcription, histone modifications (H3K4m3, H3K27m3), and DNA methylation dynamically alter nucleosome positioning and occupancy.
  • Nucleosome organization patterns around transcription start sites, exons, and transcription factor binding sites provide insights into regulatory mechanisms.
  • Nucleosome organization alone can elucidate pluripotency circuitry.

Conclusions:

  • Nucleosome organization is intricately linked to DNA sequence, transcriptional activity, and epigenetic landscapes.
  • Understanding nucleosome organization is crucial for deciphering cellular identity and the mechanisms governing pluripotency.