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Fish collagen/alginate/chitooligosaccharides integrated scaffold for skin tissue regeneration application
Pathum Chandika1, Seok-Chun Ko1, Gun-Woo Oh1
1Department of Biomedical Engineering, and Centre for Marine-Integrated Biomedical Technology (BK21 Plus), Pukyong National University, Busan 608-737, Republic of Korea; Marine-Integrated Bionics Research Center, Pukyong National University, Busan 608-737, Republic of Korea.
A novel fish collagen/alginate sponge scaffold functionalized with chitooligosaccharides (COSs) shows promise for skin tissue engineering. The FCA/COS1 scaffold exhibits optimal properties for wound healing and skin regeneration applications.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Regenerative Medicine
Background:
- Tissue-engineered skin substitutes are emerging as alternatives for functional skin creation.
- Developing advanced scaffolds is crucial for effective wound healing and skin regeneration.
Purpose of the Study:
- To develop and characterize a fish collagen/alginate (FCA) sponge scaffold functionalized with varying molecular weights of chitooligosaccharides (COSs).
- To evaluate the physicochemical, mechanical, and biological properties of the FCA/COS scaffolds for skin tissue engineering.
Main Methods:
- Fabrication of FCA/COS scaffolds using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride cross-linking.
- Analysis of cross-linking effects via Fourier transform infrared spectroscopy.
- Assessment of pore size, porosity, swelling, tensile strength, biodegradation, and cytocompatibility.
Main Results:
- Scaffold properties (blending, cross-linking, porosity, pore size) were dependent on chitooligosaccharide molecular weights (MW).
- The FCA/COS scaffolds demonstrated interconnected porous architecture (160-260μm pores, >90% porosity) and tunable swelling/retention.
- The FCA/COS1 scaffold (1-3kDa COS MW) exhibited superior cytocompatibility and overall enhanced properties.
Conclusions:
- The developed FCA/COS scaffolds possess favorable physicochemical and mechanical properties for skin tissue engineering.
- The FCA/COS1 scaffold demonstrates significant potential as a superior biomaterial for skin regeneration due to its enhanced cytocompatibility.
