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Bioreactor Design and Operational System

Bioreactor Design and Operational System

Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...

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A Multi-Cue Bioreactor to Evaluate the Inflammatory and Regenerative Capacity of Biomaterials under Flow and Stretch

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Cyclic Stretch and Perfusion Bioreactor for Conditioning Large Diameter Engineered Tissue Tubes.

Jillian B Schmidt¹, Robert T Tranquillo^2,3

¹Department of Chemical Engineering & Materials Science, University of Minnesota, Minneapolis, MN, USA.

Annals of Biomedical Engineering

|August 27, 2015

Summary

Engineered heart valve tissues showed improved remodeling when cultured in a bioreactor with porous supports. This design enhances nutrient transport and flow stimulation for faster tissue development.

Keywords:

COMSOL Multiphysics®Fibrin Tissue-engineered heart valve Transmural flow

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Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor

Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor

Published on: June 14, 2011

Area of Science:

Biomedical Engineering
Tissue Engineering
Cardiovascular Research

Background:

Developing functional engineered tissues for heart valve replacement requires robust in vitro culture systems.
Optimizing nutrient delivery and mechanical stimulation is crucial for tissue development and remodeling.

Purpose of the Study:

To compare lumenal remodeling, composition, and mechanical properties of engineered heart valve tissues cultured with different support sleeves in a novel bioreactor.
To investigate the impact of perfusion and cyclic stretch on tissue development for heart valve applications.

Main Methods:

Designed and utilized a custom cyclic stretch and perfusion bioreactor for culturing large diameter engineered tissue tubes.
Cultured dermal fibroblast-seeded fibrin gel tissues on either porated or solid latex support sleeves.
Employed computational fluid dynamics (CFD) modeling to predict fluid flow and shear stress distribution within the bioreactor.

Main Results:

Tissues cultured on porated sleeves exhibited enhanced lumenal remodeling and cellularity, particularly around pore regions.
CFD analysis indicated elevated shear stresses and transmural velocity in pore regions, correlating with improved tissue remodeling.
Cyclic stretching combined with perfusion significantly influenced tissue composition and mechanical strength.

Conclusions:

The developed bioreactor effectively promotes engineered tissue remodeling for heart valve applications.
Porous support sleeves facilitate enhanced nutrient transport and mechanical stimulation, leading to superior tissue development.
Optimized flow dynamics and mechanical cues in bioreactors are key to accelerating in vitro tissue engineering.