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Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

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Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
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Resolving Intra- and Inter-Molecular Structure with Non-Contact Atomic Force Microscopy.

Samuel Paul Jarvis1

  • 1School of Physics & Astronomy, University of Nottingham, Nottingham NG7 2RD, UK. Samuel.Jarvis@nottingham.ac.uk.

International Journal of Molecular Sciences
|August 27, 2015
PubMed
Summary

High-resolution molecular imaging is now possible using non-contact atomic force microscopy (NC-AFM) with molecularly-terminated tips. Researchers found apparent intermolecular features are often imaging artifacts due to tip-sample junction flexure.

Keywords:
NC-AFMatomic force microscopybondhydrogenintermolecularintramolecularsupramolecular

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Area of Science:

  • Surface science
  • Nanotechnology
  • Molecular imaging

Background:

  • Imaging individual molecules and their assemblies at the single-bond level presents a significant challenge.
  • Scanning probe microscopy (SPM) offers exceptional resolution, making it suitable for molecular investigations.
  • Molecularly-terminated tips enhance SPM resolution, enabling unprecedented detail.

Purpose of the Study:

  • To review landmark achievements in intramolecular resolution using non-contact atomic force microscopy (NC-AFM).
  • To focus on recent studies of molecular assemblies and observed intermolecular features.
  • To discuss the role of tip-sample junction flexure in high-resolution imaging.

Main Methods:

  • Utilizing non-contact atomic force microscopy (NC-AFM) with molecularly-terminated tips (e.g., CO, Xe, H2).
  • Analyzing imaging artifacts arising from tip-sample interactions and flexure.
  • Investigating supramolecular assemblies stabilized by intermolecular forces.

Main Results:

  • NC-AFM with molecularly-terminated tips achieves high intramolecular resolution.
  • Apparent intermolecular features in some images are identified as artifacts caused by tip-sample junction flexure.
  • Flexure critically influences the sharpness of both intra- and apparent intermolecular structural imaging.

Conclusions:

  • NC-AFM shows significant potential for resolving supramolecular assemblies with high fidelity.
  • Careful interpretation is needed to distinguish real intermolecular bonding from imaging artifacts.
  • Understanding tip-sample interactions is crucial for accurate molecular imaging.