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Updated: Apr 5, 2026

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

Published on: September 26, 2025

A comprehensive model to predict mitotic division in budding yeasts.

Sabyasachi Sutradhar¹, Vikas Yadav², Shreyas Sridhar²

¹Department of Solid State Physics, Indian Association for the Cultivation of Science, Kolkata 700032, India.

Molecular Biology of the Cell

|August 28, 2015

Summary

A computational model reveals biased nucleation of cytoplasmic microtubules is key for directional nuclear migration during yeast cell division. Distinct pathways in budding yeasts highlight microtubule dynamics essential for chromosome segregation.

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Area of Science:

Cell Biology
Systems Biology
Computational Biology

Background:

High-fidelity chromosome segregation is crucial for cell division.
Mitotic events involve complex, interdependent interactions.
Budding yeasts (Ascomycota and Basidiomycota) serve as model organisms.

Purpose of the Study:

To develop a minimal computational model of mitotic events in budding yeasts.
To comprehend the interdependence of cellular processes during mitosis.
To identify key factors driving chromosome segregation, spindle alignment, and nuclear migration.

Main Methods:

Systems biology approach to model construction.
Development of a minimal computational model.
Validation against experimental observations of spindle alignment, nuclear migration, and microtubule dynamics.

Main Results:

The model accurately reproduces experimental data for yeast cell division.
Biased nucleation of cytoplasmic microtubules (cMTs) is identified as essential for directional nuclear migration.
Two distinct pathways involving cMTs and cortical dyneins differentiate nuclear migration and spindle orientation between fungal phyla.
The model predicts the roles of specific microtubule classes in chromosome segregation.

Conclusions:

Biased cytoplasmic microtubule nucleation is a critical determinant of directional nuclear migration.
Divergent molecular pathways govern nuclear migration and spindle orientation in Ascomycota and Basidiomycota.
The developed model provides a versatile tool for simulating perturbations in mitotic cell division.