Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of Regeneration and Repair01:19

Overview of Regeneration and Repair

5.9K
Regeneration and repair processes are critical in healing damages caused by injury, disease, and aging. In regeneration, the damaged tissue is entirely replaced with new growth that restores the original architecture and function. In contrast, tissue repair usually results in a fixed tissue architecture involving scar formation. Scars generally do not reestablish tissue function and may also exhibit structural abnormalities at the injury site.
Regeneration
All animals have varying degrees of...
5.9K
Whole Body Regeneration01:33

Whole Body Regeneration

4.6K
Regeneration is the process of restoring injured or lost tissues, organs, or body parts. While simpler organisms generally show greater ability to regenerate their whole body, few complex animals show similarly exceptional regeneration. For example, planarian flatworms have a unique regenerative potential making them a popular study organism among biologists to understand the mechanisms of whole body regeneration. Other organisms, such as hydra, also show extreme regeneration potential;...
4.6K
Stem Cell Therapy for Tissue Regeneration01:21

Stem Cell Therapy for Tissue Regeneration

4.9K
Stem cell therapy is a method used in regenerative medicine to repair and restore function to damaged tissues and organs. Stem cells have the potential to proliferate and differentiate into various tissue types, making them ideal candidates for tissue regeneration. For example, hematopoietic stem cell transplants are commonly used in blood cancer treatment to replenish damaged bone marrow and restore healthy blood cells.
Types of Stem Cells used in Stem Cell Therapy
The two main cell...
4.9K
Teeth01:15

Teeth

2.3K
The formation of teeth, also known as odontogenesis, is a complex process that begins in utero, around the sixth week of embryonic development. There are three stages to this process: the bud stage, the cap stage, and the bell stage.
In the bud stage, the tooth germ (an aggregation of cells) starts to form in the developing jawbone. During the cap stage, the tooth germ differentiates into enamel organ, dental papilla, and dental sac, which will later develop into the tooth's enamel, dentin...
2.3K
Tooth Anatomy01:21

Tooth Anatomy

2.9K
The human tooth enables us to eat a variety of foods, speak clearly, and even aid in shaping our faces. Teeth are composed of various elements that work together. Here's a detailed look at the anatomy of a human tooth.
The Crown, Neck, and Root
The visible part of the tooth is referred to as the crown. It's covered by enamel, the hardest substance in the human body. The crown is uniquely shaped for each type of tooth, allowing for different functions such as cutting, tearing, or...
2.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Safety of percutaneous versus transjugular liver biopsy: A propensity score matched analysis.

European journal of radiology·2020
Same author

Dijet Resonance Search with Weak Supervision Using sqrt[s]=13  TeV pp Collisions in the ATLAS Detector.

Physical review letters·2020
Same author

CP Properties of Higgs Boson Interactions with Top Quarks in the tt[over ¯]H and tH Processes Using H→γγ with the ATLAS Detector.

Physical review letters·2020
Same author

Search for Heavy Higgs Bosons Decaying into Two Tau Leptons with the ATLAS Detector Using pp Collisions at sqrt[s]=13  TeV.

Physical review letters·2020
Same author

Reply by Authors.

The Journal of urology·2019
Same author

Beam Energy and Centrality Dependence of Direct-Photon Emission from Ultrarelativistic Heavy-Ion Collisions.

Physical review letters·2019
Same journal

Gold Nanoparticles Enhance the Antibacterial and Osteogenic Properties of Polyetheretherketone.

Journal of dental research·2026
Same journal

Periodontitis-Aggravated Diabetic Kidney Disease with Altered Glycolysis.

Journal of dental research·2026
Same journal

Response to Letter to Editor: "Estimating the Individualized Effect of Tooth Extraction before Radiotherapy on Osteoradionecrosis Using Causal Machine Learning".

Journal of dental research·2026
Same journal

Reorienting Oral Health Promotion through Systems Thinking.

Journal of dental research·2026
Same journal

<i>Porphyromonas gingivalis</i>-Induced NETs Mediate Neuroinflammation via TLR4 Activation.

Journal of dental research·2026
Same journal

Oral Burden of Sjögren Disease: A Systematic Review and Meta-analysis.

Journal of dental research·2026
See all related articles

Related Experiment Video

Updated: Apr 5, 2026

Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice
07:07

Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice

Published on: January 12, 2017

12.0K

Pulp-dentin Regeneration: Current State and Future Prospects.

Y Cao1, M Song1, E Kim2

  • 1School of Dentistry, UCLA, Los Angeles, CA, USA.

Journal of Dental Research
|August 28, 2015
PubMed
Summary
This summary is machine-generated.

Regenerative endodontics aims to restore pulp function in infected teeth. Induced mesenchymal stem cells (iMSCs) from keratinocytes offer a promising alternative for pulp-dentin regeneration.

Keywords:
biocompatible materialscell- and tissue-based therapydental pulp calcificationendodonticsepithelial-mesenchymal transitionstem cells

More Related Videos

Author Spotlight: Advancing Tissue Regeneration and Disease Modeling with Dental Pulp Stem Cells
03:45

Author Spotlight: Advancing Tissue Regeneration and Disease Modeling with Dental Pulp Stem Cells

Published on: May 5, 2023

3.5K
Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods
14:52

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Published on: November 24, 2012

27.4K

Related Experiment Videos

Last Updated: Apr 5, 2026

Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice
07:07

Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice

Published on: January 12, 2017

12.0K
Author Spotlight: Advancing Tissue Regeneration and Disease Modeling with Dental Pulp Stem Cells
03:45

Author Spotlight: Advancing Tissue Regeneration and Disease Modeling with Dental Pulp Stem Cells

Published on: May 5, 2023

3.5K
Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods
14:52

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Published on: November 24, 2012

27.4K

Area of Science:

  • Dental research
  • Regenerative medicine
  • Stem cell biology

Background:

  • Regenerative endodontics seeks to restore pulp vitality and function in necrotic teeth.
  • Current approaches like revascularization show limitations in achieving complete pulp-dentin regeneration.
  • Mesenchymal stem cells (MSCs) are crucial for cell-based endodontic regeneration, but clinical translation faces challenges like cell aging and availability.

Purpose of the Study:

  • To explore induced mesenchymal stem cells (iMSCs) as an alternative cell source for endodontic regeneration.
  • To investigate the potential of iMSCs derived from keratinocytes for pulp-dentin regeneration.
  • To overcome limitations associated with autologous pulpal MSCs.

Main Methods:

  • Developed iMSCs from primary human keratinocytes via epithelial-mesenchymal transition.
  • Utilized modulation of epithelial plasticity genes, including p63 isoforms (overexpression of ΔNp63α or transient knockdown via siRNA).
  • Assessed stem cell characteristics and potential for transdifferentiation into odontoblasts.

Main Results:

  • Successfully generated iMSCs from keratinocytes exhibiting stem cell properties.
  • Demonstrated a simplified protocol for iMSC generation using siRNA knockdown of p63 isoforms, avoiding viral vectors.
  • iMSCs show potential as an alternative cell source for patients lacking endogenous MSCs.

Conclusions:

  • Induced MSCs (iMSCs) represent a viable alternative cell source for endodontic regeneration.
  • Further research is needed to confirm the potency and transdifferentiation capacity of iMSCs into functional odontoblasts.
  • iMSC technology may address challenges in clinical translation of regenerative endodontics, particularly regarding cell source and safety.