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Updated: Apr 5, 2026

Fabrication of the Thermoplastic Microfluidic Channels
Published on: February 3, 2008
Stepped gradients on polymeric monolithic columns by photoinitiated grafting
Sinéad Currivan1, Damian Connolly2, Brett Paull1,3
1Australian Centre for Research on Separation Science, School of Physical Sciences, University of Tasmania, Sandy Bay, Hobart, Tasmania, Australia.
Abstract:
The surface of polymethacrylate monoliths was functionalized by a post-polymerization modification, by means of a novel photo-initiated graft procedure where the charged monomer, sulfopropyl methacrylate, was controllably grafted stepwise, i.e. with incremental graft energies. The grafting approach was optimized using scanning capacitively coupled contactless conductivity detection. The effect of the localized ion exchange capacity and resultant gradient stationary phase upon ion-exchange chromatographic retention, selectivity, and performance was investigated, and compared to a homogeneously grafted (isotropic) column. The gradient column provided reduced peak widths at half height for both cationic analytes, with a reduction of 34 and 33%, respectively, when compared to the isotropic column.
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