Related Experiment Video
Updated: Apr 5, 2026

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
Published on: April 6, 2012
MicroRNA-10b promotes migration and invasion through Hoxd10 in human gastric cancer
Yuan-Yu Wang1, Li Li2, Zai-Yuan Ye3
1Departments of Gastrointestinal Surgery and Pathology, Zhejiang Provincial People's Hospital, Hangzhou, 310014, People's Republic of China. lywyy1979@126.Com.
Background:
This study aims to investigate the effect of miR-10b overexpression on cancer cell proliferation, migration, invasion, and Hoxd10 expression.
Methods:
The effect of miR-10b on proliferation, migration, and invasion of MKN-28, BGC-823, and SGC-7901 cells and the expression of Hoxd10 protein in SGC-7901 and BGC-823 cells were detected following transfection of miR-10b inhibitor or Negative Control B. Expression of Hoxd10 protein in 436 paraffin-embedded cancer tissues was also investigated.
Results:
miR-10b was significantly upregulated in AGS, MKN-28, BGC-823, HCG-27, SGC-7901, and MKN-45 cell lines, miR-10b inhibitor significantly inhibited proliferation and migration of MKN-45, BGC-823 and SGC-7901 cells 48 h after transfection, while Hoxd10 protein in these cells lines had increased 72 h after transfection. Hoxd10 was highly expressed in gastric cancer and correlated with size of tumor, Lauren classification, depth of invasion, lymph node and distant metastasis, Tumor-Node-Metastasis (TNM) stage, and prognosis.
Conclusions:
miR-10b promotes migration and invasion through Hoxd10 in human gastric cancer cell lines and may play an important role in tumorigenesis, progression, and prognosis.
Related Concept Videos
Cancer Cell Migration through Invadopodia
MicroRNAs
MicroRNAs
Cell Migration
Cell Migration
Small GTPases - Ras and Rho
Three regulatory proteins control their activity:

