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Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...

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Updated: Apr 4, 2026

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

Published on: June 16, 2016

Advances in microfluidic platforms for analyzing and regulating human pluripotent stem cells.

Tongcheng Qian¹, Eric V Shusta¹, Sean P Palecek¹

¹Department of Chemical and Biological Engineering, University of Wisconsin-Madison, 1415 Engineering Drive, Madison, WI 53706, USA.

Current Opinion in Genetics & Development

|August 28, 2015

Summary

Microfluidics precisely control cell microenvironments, advancing human pluripotent stem cell (hPSC) research for disease modeling and drug screening. These advanced systems enable better understanding of hPSC fate regulation.

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Area of Science:

Biotechnology
Stem Cell Biology
Microfluidics

Background:

Microfluidic devices offer precise control over cellular microenvironments.
Human pluripotent stem cells (hPSCs) are crucial for regenerative medicine and disease modeling.
Understanding hPSC regulation is key to harnessing their therapeutic potential.

Purpose of the Study:

To review recent advances in microfluidic applications for studying hPSC regulation.
To highlight the use of microfluidics in controlling hPSC fates and microenvironments.
To discuss microfluidic systems for in vitro modeling and screening using hPSCs.

Main Methods:

Utilizing submillimeter scale flow control for high-resolution spatial and temporal manipulation of the cellular microenvironment.
Implementing microfluidics to control paracrine and juxtracrine signaling pathways.
Integrating microfluidic cell culture systems with actuators and sensors for biosensor applications.

Main Results:

Microfluidics enable detailed elucidation of microenvironmental factors regulating hPSC fate.
Microfluidic platforms facilitate the creation of multi-cellular organs-on-a-chip models.
Integration with sensors allows for high-density cell-based biosensor arrays for screening.

Conclusions:

Microfluidics are powerful tools for understanding hPSC regulation mechanisms.
Microfluidic technologies are essential for realizing the potential of hPSCs in in vitro modeling and drug discovery.
Advances in microfluidics pave the way for sophisticated cell-based screening applications.