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Updated: Apr 4, 2026

Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis
Published on: January 31, 2025
[Effect of As₂O₃on Hedgehog Pathway in Chronic Myeloid Leukemia Cells]
Xiao-Wei He1, Wei Yan1, Wei Yang1
1Department of Hematology, Shengjing Hospital Affiliated to China Medical University, Shenyang 110022, Liaoning Province, China.
Objective:
To explore the effect of As₂O₃on Hedgehog pathway in chronic myeloid leukemia (CML) cells.
Methods:
The apoptosis of K562 cells was detected by MTT method and flow cytometry; the expressions of PTCH and SMO protein and mRNA in Hedgehog pathway were determined by Western blot and real-time PCR, respectively. Retults: The As₂O₃ could induce the apoptosis of K562 cells with optimal concentration 2 µmol/L and optimal time 24 hours. The expressions of PTCH and SMO protein and mRNA in Hedgehog pathway of K562 cells treated with As₂O₃at optimal concentration and optimal time were down-regulated.
Conclusion:
The As₂O₃can down-regulate the expression of PTCH and SMO in Hedgehog pathway.
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