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Published on: January 2, 2013
[Identification of B (A) Blood Group and Blood Transfusion for patients with B (A) Blood Group]
Chao Wang1, Su-Ping Li1, Xue-Zhong Wu1
1Anhui Provincial Blood Center, Department of Blood Transfusion, Hefei Blood Center, Hefei 230031, Anhui Province, China.
Objective:
To investigate the serological and molecular biological identification of B(A) blood group and its reasonable method of blood transfusion for patient with B(A) blood group.
Methods:
The blood group of patient was detected by serological method, at the some time, the genotype of patient was detected by using the ABO-TYPE Variant kit and sequence analysis of 6 and 7 exons in ABO gene; the washed O red blood cells were used to cross matching blood of difficultly matching blood by the three step analysis method.
Results:
The A weak and B strong agglutination were found in positive type, and A1C(3+), BC(-) were observed in negative type; the molecular biological identification showed B(A)04, 640 A > G; the matching blood main side of washed O red blood cells displayed no agglutination.
Conclusion:
The identification and analysis of rare blood or subtype should be very careful; if necessary, the molecular biological detection should carried out; the blood transfusion for patient with rate blood group or subtype should be safe, correct and reasonable.
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