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Related Concept Videos

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Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...
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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
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Related Experiment Video

Updated: Apr 4, 2026

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
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Single cell nanobiosensors for dynamic gene expression profiling in native tissue microenvironments.

Shue Wang1, Reza Riahi2, Na Li3

  • 1Department of Aerospace and Mechanical Engineering, The University of Arizona, Tucson, AZ, 85721, USA.

Advanced Materials (Deerfield Beach, Fla.)
|August 29, 2015
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel gold nanorod-locked nucleic acid nano-biosensor for real-time gene expression analysis in living cells and tissues. This advanced biosensor enables dynamic, single-cell level monitoring in various biological samples.

Keywords:
biosensorslocked nucleic acidsnanoparticlessingle cellstissue microenvironments

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Area of Science:

  • Biotechnology
  • Nanotechnology
  • Molecular Biology

Background:

  • Gene expression analysis is crucial for understanding cellular function and disease.
  • Existing methods for dynamic gene expression monitoring often lack single-cell resolution or in vivo applicability.

Purpose of the Study:

  • To develop and validate a novel nano-biosensor for dynamic, single-cell gene expression analysis.
  • To demonstrate the utility of the nano-biosensor in living cells and diverse tissue types.

Main Methods:

  • Fabrication of a gold nanorod-locked nucleic acid (LNA) nano-biosensor.
  • Utilizing the nano-biosensor for endocytic delivery into target cells.
  • Dynamic monitoring of gene expression at the single-cell level in vitro and in vivo.

Main Results:

  • Successful development of a gold nanorod-LNA nano-biosensor.
  • Demonstrated endocytic delivery and efficient gene expression monitoring in human umbilical cord endothelial cells.
  • Validated single-cell gene expression analysis in mouse skin, retina, and cornea tissues.

Conclusions:

  • The developed nano-biosensor is effective for dynamic, single-cell gene expression analysis.
  • This technology offers a powerful tool for studying gene regulation in living systems.
  • Potential applications in diagnostics and therapeutic development for gene-related diseases.