Isolation and characterization of genes coding for proteins involved in the cytolysis by Listeria ivanovii

We established a library of chromosomal DNA of Listeria ivanovii in the pTZ19R plasmid system, using Escherichia coli DH5 alpha as the host. One recombinant clone reacted strongly with a polyclonal antiserum raised against the listeriolysin O and a second exoprotein (24kDa) of L. ivanovii, which is most probably also involved in cytolytic processes. The recombinant E. coli clone may contain part of the listeriolysin O gene of L. ivanovii.