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Gene probes for the detection of Listeria spp
M Leimeister-Wächter1, T Chakraborty
1Institute of Genetics and Microbiology, University of Würzburg, FRG.
Abstract:
Four cloned genes of Listeria monocytogenes coding for listeriolysin O, beta-haemolysin, CAMP-factor and a DTH-inducing protein (DTH-18) were used as gene probes in DNA-DNA hybridization with different listerial references strains. The fragment encoding for DTH-18 is the only probe reacting specifically with pathogenic strains of L. monocytogenes and Listeria ivanovii.