[Disaccharidases: 25 years later]

This report shows, that the major factors involved were histological damage of the mucosa and the etiological diagnosis. Other factors (bacterial overgrowth, malnutrition, etc.), added to the previous referred, increase the oligosaccharidases alteration, yielding statistically significant values referring to healthy subjects. The oligosaccharidases activity were significantly decreased to referring to healthy subjects (p less than 0.001) in the IC1.o and 2.o that have not histological damage, in the DGR, IPLV and coeliac disease with AS of the mucosa and in the SPG that present APM. On the other hand, the analytical methodology with its different biochemical basis could evidence very various values and there is always important to remark the reference normal values in each laboratory.