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Studies on the tissue specificity of a circulating renotropic factor.

W Hernandez, H Goldin, M Shivers

Acta Physiologica Latino Americana

|January 1, 1979

Summary

Sera from nephrectomized rats stimulate kidney cell DNA synthesis. A renal tissue factor appears to activate this renotropic substance, enabling it to act on liver cells as well.

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Area of Science:

Nephrology
Molecular Biology
Biochemistry

Background:

Sera from unilaterally nephrectomized rats stimulate DNA synthesis in renal cortex tissue.
This effect was previously considered specific to kidney tissue.
A renal tissue factor was identified that enhances the action of circulating renotropic factors.

Purpose of the Study:

To investigate the role of the renal tissue factor in the specificity of serum-stimulated DNA synthesis.
To determine if the renal tissue factor can enable non-renal tissues to respond to renotropic factors.

Main Methods:

Incubation of rat renal cortex slices with sera from nephrectomized rats.
Incubation of rat liver, spleen, and lung slices with sera from nephrectomized rats.
Addition of a renal tissue factor to liver slices incubated with sera from uninephrectomized rats.
Measurement of 3H-Thymidine incorporation into DNA as an indicator of cell proliferation.

Main Results:

Sera from nephrectomized rats stimulated 3H-Thymidine incorporation into renal cortex DNA.
These sera did not stimulate isotope incorporation in liver, spleen, or lung tissue.
The addition of the renal tissue factor and sera from uninephrectomized rats stimulated 3H-Thymidine incorporation into liver slices.

Conclusions:

The renal tissue factor plays a crucial role in activating circulating renotropic substances.
This activation mechanism explains the observed specificity of renotropic factors for renal tissue.
The renal tissue factor can enable non-renal tissues, such as the liver, to respond to renotropic stimulation.

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