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Following Cell-fate in E. coli After Infection by Phage Lambda
Published on: October 14, 2011
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Phage L5 integrating vectors are present within the Mycobacterial Cell in an equilibrium between integrated and
1Basic Medical Sciences, College of Osteopathic Medicine, Western University of Health Sciences, 309 E. Second St. Pomona CA 91766.
Cancer Therapy
|August 29, 2015
Abstract:
Integrating mycobacterial plasmids containing the phage L5 attachment site (attP) are able to insert into the mycobacterial chromosome attB site. Plasmids containing the attP site and chromosome containing the attB site are present in equilibrium between the inserted and the excised states in the presence of the phage L5 integrase.
Insights
Mycobacterial plasmids with the phage L5 attachment site (attP) can integrate into the attB site on the chromosome. The phage L5 integrase maintains an equilibrium between plasmid insertion and excision states.
Area of Science:
- Microbiology
- Molecular Biology
- Genetics
Background:
- Mycobacterial plasmids are extrachromosomal DNA elements.
- Site-specific recombination is a key mechanism in genetic engineering.
- Phage L5 integrase mediates recombination between attP and attB sites.
Purpose of the Study:
- To investigate the integration mechanism of mycobacterial plasmids containing the phage L5 attachment site (attP) into the mycobacterial chromosome.
- To understand the role of phage L5 integrase in regulating plasmid-chromosome interactions.
Main Methods:
- Utilizing plasmids with the attP site and mycobacterial strains with the attB site.
- Employing phage L5 integrase for recombination studies.
- Analyzing the equilibrium between inserted and excised plasmid states.
Main Results:
- Demonstrated successful integration of mycobacterial plasmids into the chromosomal attB site.
- Showcased the dynamic equilibrium between integrated and excised plasmid forms.
- Confirmed the essential role of phage L5 integrase in this process.
Conclusions:
- Phage L5 integrase facilitates site-specific integration of plasmids into the mycobacterial chromosome.
- The integration process is reversible, maintaining an equilibrium influenced by integrase activity.
- This system offers potential for stable genetic manipulation in mycobacteria.
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