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Updated: Apr 4, 2026
Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
Published on: October 1, 2016
Fluorescence resonance energy transfer-based ratiometric fluorescent assay for highly sensitive and selective
Meijuan Liang1, Yonglei Chen, Haijuan Zhang
1State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China. chenxg@lzu.edu.cn.
Abstract:
A novel and effective ratiometric fluorescence strategy was developed for rapidly, sensitively and selectively probing sulfide anions (S(2-)). A dual-emission nanosensor was prepared by covalently attaching fluorescent carbon nanoparticles (CNPs) to gold nanoclusters (Au NCs), triggering the sensing mechanism of fluorescence resonance energy transfer (FRET) from CNPs (donor) to Au NCs (acceptor). Once S(2-) was added, considerable fluorescence recovery of CNPs and quenching of Au NCs were observed due to the inhibition of FRET progress via the formation of Au2S. The ratiometric probe showed good, specific S(2-) sensing behavior and high sensitivity with a detection limit of 18 nM. Significantly, the assay was successfully employed to determine the S(2-) content in biological and water samples, presenting immense promise in the biological and environmental fields.
