Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Lipid composition in the classification of Rhodococcus equi.

M D Barton1, M Goodfellow, D E Minnikin

  • 1Veterinary Research Institute, Parkville, Victoria, Australia.

Zentralblatt Fur Bakteriologie : International Journal of Medical Microbiology
|December 1, 1989
PubMed
Summary

Lipid analysis of Rhodococcus equi and related species reveals distinct fatty acid, menaquinone, and polar lipid profiles. These findings support Rhodococcus equi as a unique taxospecies within the Rhodococcus genus.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Primary preputial reconstruction following surgical excision of cutaneous mast cell tumours without penile amputation in eight dogs.

New Zealand veterinary journal·2024
Same author

Treatment outcomes of laryngectomy compared to non-surgical management of T<sub>3</sub> laryngeal carcinomas: a 10-year multicentre audit of 179 patients in the northeast of England.

The Journal of laryngology and otology·2021
Same author

Extreme environments: microbiology leading to specialized metabolites.

Journal of applied microbiology·2019
Same author

Relation between facial fractures and socioeconomic deprivation in the north east of England.

The British journal of oral & maxillofacial surgery·2019
Same author

Estimation of brain network ictogenicity predicts outcome from epilepsy surgery.

Scientific reports·2016
Same author

Charting stormy waters: A commentary on the nomenclature of the equine pathogen variously named Prescottella equi, Rhodococcus equi and Rhodococcus hoagii.

Equine veterinary journal·2015

Area of Science:

  • Microbiology
  • Lipidomics
  • Chemotaxonomy

Background:

  • Rhodococcus equi is an important animal and human pathogen.
  • Understanding the lipid composition of Rhodococcus species is crucial for their classification and identification.

Purpose of the Study:

  • To characterize the fatty acid, menaquinone, and polar lipid profiles of Rhodococcus equi and related taxa.
  • To evaluate the utility of lipid data in the taxonomic differentiation of Rhodococcus species.

Main Methods:

  • Gas chromatography-mass spectrometry (GC-MS) for fatty acid analysis.
  • High-performance liquid chromatography (HPLC) for menaquinone and polar lipid analysis.
  • Pyrolysis-GC-MS for mycolic acid analysis.

Main Results:

Related Experiment Videos

  • Rhodococcus equi strains exhibited characteristic lipid profiles, including specific fatty acid compositions, dihydrogenated menaquinones with eight isoprene units, and distinct polar lipid patterns.
  • Mycolic acids ranged from C24 to C48 with 0-4 double bonds.
  • Lipid profiles of other analyzed strains were consistent with their assignment to the genus Rhodococcus, including Rhodococcus sputi.

Conclusions:

  • The distinct lipid composition of Rhodococcus equi supports its classification as a separate taxospecies within the Rhodococcus genus.
  • Lipidomic analysis provides valuable chemotaxonomic markers for differentiating Rhodococcus species.