Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nuclear Export of mRNA02:31

Nuclear Export of mRNA

9.2K
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
9.2K
Nuclear Export01:42

Nuclear Export

5.2K
The nucleus restricts several proteins within and allows others to pass. The restricted proteins possess a nuclear retention sequence or NRS, anchoring them to the nuclear lamins and preventing their transport to the cytosol. The non-restricted proteins, after their synthesis, are transported to their site of action, such as the cytosol or other organelles, with the help of nuclear export signals or NES.
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
5.2K
Overview of Exosomes01:36

Overview of Exosomes

3.9K
Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
3.9K
Exon Recombination02:32

Exon Recombination

4.3K
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon...
4.3K
RNA-seq03:21

RNA-seq

12.6K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
12.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mapping of in vivo cleavage sites uncovers a major role for yeast RNase III in regulating protein-coding genes.

eLife·2026
Same author

Microbial signals in primary and metastatic brain tumors.

Nature medicine·2025
Same author

A eukaryote without tRNA introns.

RNA (New York, N.Y.)·2025
Same author

Mapping of in vivo cleavage sites uncovers a major role for yeast RNase III in regulating protein-coding genes.

bioRxiv : the preprint server for biology·2025
Same author

A eukaryote without tRNA introns.

bioRxiv : the preprint server for biology·2025
Same author

The highly conserved intron of tyrosine tRNA is critical for <sup>m1</sup>A58 modification and controls the integrated stress response.

Proceedings of the National Academy of Sciences of the United States of America·2025

Related Experiment Video

Updated: Apr 4, 2026

Isolation and Characterization of RNA-Containing Exosomes
09:43

Isolation and Characterization of RNA-Containing Exosomes

Published on: January 9, 2012

100.8K

Gateway Arch to the RNA Exosome.

Jillian S Losh1, Ambro van Hoof1

  • 1Department of Microbiology and Molecular Genetics, University of Texas Health Science Center-Houston and The University of Texas Graduate School of Biomedical Sciences, 6431 Fannin Street, MSB 1.212, Houston, TX 77030, USA.

Cell
|August 29, 2015
PubMed
Summary

Researchers have uncovered how the RNA exosome, a cellular complex, identifies specific RNA molecules for degradation. This finding clarifies a key mechanism in RNA processing and cellular regulation.

More Related Videos

Isolation of Next-Generation Gene Therapy Vectors through Engineering, Barcoding, and Screening of Adeno-Associated Virus AAV Capsid Variants
09:20

Isolation of Next-Generation Gene Therapy Vectors through Engineering, Barcoding, and Screening of Adeno-Associated Virus AAV Capsid Variants

Published on: October 18, 2022

5.5K
RNA Next-Generation Sequencing and a Bioinformatics Pipeline to Identify Expressed LINE-1s at the Locus-Specific Level
11:04

RNA Next-Generation Sequencing and a Bioinformatics Pipeline to Identify Expressed LINE-1s at the Locus-Specific Level

Published on: May 19, 2019

10.6K

Related Experiment Videos

Last Updated: Apr 4, 2026

Isolation and Characterization of RNA-Containing Exosomes
09:43

Isolation and Characterization of RNA-Containing Exosomes

Published on: January 9, 2012

100.8K
Isolation of Next-Generation Gene Therapy Vectors through Engineering, Barcoding, and Screening of Adeno-Associated Virus AAV Capsid Variants
09:20

Isolation of Next-Generation Gene Therapy Vectors through Engineering, Barcoding, and Screening of Adeno-Associated Virus AAV Capsid Variants

Published on: October 18, 2022

5.5K
RNA Next-Generation Sequencing and a Bioinformatics Pipeline to Identify Expressed LINE-1s at the Locus-Specific Level
11:04

RNA Next-Generation Sequencing and a Bioinformatics Pipeline to Identify Expressed LINE-1s at the Locus-Specific Level

Published on: May 19, 2019

10.6K

Area of Science:

  • Molecular Biology
  • Cellular Biology
  • Biochemistry

Background:

  • The RNA exosome is a crucial multi-subunit complex responsible for degrading various RNA species within cells.
  • Understanding the specificity of RNA exosome targeting is essential for comprehending gene expression regulation and cellular homeostasis.

Purpose of the Study:

  • To elucidate the mechanisms by which the RNA exosome recognizes and selects distinct subsets of target RNAs for degradation.
  • To fill a significant knowledge gap in the field of RNA processing and surveillance.

Main Methods:

  • Investigated RNA-binding proteins and their interactions with the RNA exosome.
  • Utilized biochemical assays and genetic approaches to analyze RNA exosome function.
  • Characterized the structural and functional basis of RNA recognition by the exosome complex.

Main Results:

  • Identified specific factors that mediate the recognition of distinct RNA targets by the RNA exosome.
  • Demonstrated how these factors contribute to the recruitment of the exosome to specific RNA molecules.
  • Provided molecular insights into the specificity of RNA degradation pathways.

Conclusions:

  • The study reveals key mechanisms governing RNA exosome substrate selection.
  • This work advances our understanding of RNA turnover and its role in cellular processes.
  • The findings have implications for understanding diseases related to RNA dysregulation.