Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chromatin Packaging01:32

Chromatin Packaging

20.3K
Each human somatic cell contains 6 billion base pairs of DNA. Each base pair is 0.34 nm long, meaning each diploid cell contains a staggering 2 meters of DNA. This long DNA strand is packed inside a nucleus measuring only 10-20 microns in diameter with the help of specialized DNA-binding proteins called histones. Together they form a compact DNA-protein complex called chromatin. The chromatin is further compacted into higher-order structures. The highest level of compaction is achieved during...
20.3K
Chromatin Packaging02:21

Chromatin Packaging

23.3K
Each human somatic cell contains 6 billion base-pairs of DNA. Each base-pair is 0.34 nm long, which means that each diploid cell contains a staggering 2 meters of DNA. How is such a long DNA strand packed inside a nucleus measuring only 10 - 20 microns in diameter? 
The chromatin
In combination with specialized DNA binding protein called Histones, the DNA double helix forms a compact DNA: protein complex called chromatin. The chromatin itself is further compacted into higher-order...
23.3K
Chromatin Packaging02:21

Chromatin Packaging

10.3K
10.3K
Chromatin Position Affects Gene Expression02:35

Chromatin Position Affects Gene Expression

25.2K
Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
25.2K
Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

10.0K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
10.0K
Duplication of Chromatin Structure02:05

Duplication of Chromatin Structure

7.6K
The process of chromosome duplication during cell division requires genome-wide disruption and re-assembly of chromatin. The chromatin structure must be accurately inherited, reassembled, and maintained in the daughter cells to ensure lineage propagation.
The basic unit of the chromatin is the nucleosome, consisting of DNA wrapped around octameric histone proteins and short stretches of linker DNA separating individual nucleosomes. The histone proteins within the nucleosome have their...
7.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Characterization of induced cohesin loop extrusion trajectories in living cells.

Nature genetics·2025
Same author

Reactivation of developmentally silenced globin genes through forced linear recruitment of remote enhancers.

Blood·2025
Same author

Identification of a SNAI1 enhancer RNA that drives cancer cell plasticity.

Nature communications·2025
Same author

Long-range enhancer-controlled genes are hypersensitive to regulatory factor perturbations.

Cell genomics·2025
Same author

Two unrelated distal genes activated by a shared enhancer benefit from localizing inside the same small topological domain.

Genes & development·2025
Same author

A cis-regulatory element regulates ERAP2 expression through autoimmune disease risk SNPs.

Cell genomics·2024

Related Experiment Video

Updated: Apr 4, 2026

Associated Chromosome Trap for Identifying Long-range DNA Interactions
14:49

Associated Chromosome Trap for Identifying Long-range DNA Interactions

Published on: April 23, 2011

15.1K

A Long-Distance Chromatin Affair.

Annette Denker1, Wouter de Laat1

  • 1Hubrecht Institute-KNAW and University Medical Center Utrecht, 3584 CT Utrecht, the Netherlands.

Cell
|August 29, 2015
PubMed
Summary
This summary is machine-generated.

Changes in transcription factor binding sites alter chromatin composition both nearby and far away. These coordinated changes are driven by spatial chromatin interactions forming regulatory modules in the human genome.

More Related Videos

Capturing Chromosome Conformation Across Length Scales
10:15

Capturing Chromosome Conformation Across Length Scales

Published on: January 20, 2023

4.2K
Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes
07:41

Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes

Published on: October 2, 2017

9.0K

Related Experiment Videos

Last Updated: Apr 4, 2026

Associated Chromosome Trap for Identifying Long-range DNA Interactions
14:49

Associated Chromosome Trap for Identifying Long-range DNA Interactions

Published on: April 23, 2011

15.1K
Capturing Chromosome Conformation Across Length Scales
10:15

Capturing Chromosome Conformation Across Length Scales

Published on: January 20, 2023

4.2K
Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes
07:41

Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes

Published on: October 2, 2017

9.0K

Area of Science:

  • Genomics
  • Molecular Biology
  • Epigenetics

Background:

  • Transcription factor binding sites are crucial for gene regulation.
  • Chromatin composition changes can affect gene expression.
  • Long-range genomic interactions play a role in genome organization.

Purpose of the Study:

  • To investigate the mechanisms linking transcription factor binding site changes to chromatin composition alterations.
  • To explore whether these alterations occur locally and at distant genomic sites.
  • To identify the role of spatial chromatin interactions in mediating these effects.

Main Methods:

  • Analysis of transcription factor binding sequences.
  • Chromatin composition profiling.
  • Assessment of long-range chromatin interactions (e.g., using Hi-C).

Main Results:

  • Changes in transcription factor binding sequences correlate with altered chromatin composition.
  • This concordance extends to genomic regions hundreds of kilobases away from the binding sites.
  • Spatial chromatin interactions were identified as the mediating mechanism.

Conclusions:

  • Spatial chromatin interactions are key mediators of coordinated chromatin changes across the genome.
  • These interactions form functional regulatory modules within the human genome.
  • Understanding these interactions is vital for deciphering gene regulation and genome organization.