Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Scale-Up Processes01:14

Scale-Up Processes

73
The scale-up of microbial fermentation processes is essential in industrial biotechnology, allowing the transition from laboratory-scale experiments to commercial-scale production while aiming to maintain product yield and quality. This process requires meticulous adjustment of equipment design, process parameters, and contamination control strategies to accommodate increasing culture volumes.At the laboratory scale, cultures are typically maintained in 1 to 10-liter glass or autoclavable...
73
Upstream Processing01:27

Upstream Processing

77
Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
77
Bioreactor Design and Operational System01:29

Bioreactor Design and Operational System

86
Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...
86
Bioreactor Controls-I01:28

Bioreactor Controls-I

64
Maintaining optimal conditions within fermenters is essential for maximizing microbial productivity and ensuring process efficiency. This lesson focuses on key parameters—temperature, foam, pH, carbon dioxide, oxygen, and pressure—and their precise measurement and control strategies in fermentation systems.Temperature ControlTemperature regulation is critical due to the exothermic nature of many fermentation processes. In small laboratory fermenters, temperature is commonly...
64
Bioreactor Controls-II01:18

Bioreactor Controls-II

56
In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the...
56
Bioreactor Controls-III01:22

Bioreactor Controls-III

53
Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
53

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Strategies for removing two "problematic" host cell proteins, using an "end-to-end" approach.

Biotechnology progress·2026
Same author

A precision gene-engineered B cell medicine producing sustained levels of active factor IX for hemophilia B therapy.

Molecular therapy : the journal of the American Society of Gene Therapy·2025
Same author

CD200R1 immune checkpoint blockade by the first-in-human anti-CD200R1 antibody 23ME-00610: molecular mechanism and engineering of a surrogate antibody.

mAbs·2024
Same author

Type 2 hypersensitivity disorders, including systemic lupus erythematosus, Sjögren's syndrome, Graves' disease, myasthenia gravis, immune thrombocytopenia, autoimmune hemolytic anemia, dermatomyositis, and graft-versus-host disease, are THαβ-dominant autoimmune diseases.

Virulence·2024
Same author

Development of bioreactor scale-down model using orthogonal projections to latent structures method and CO<sub>2</sub> supplementation.

Biotechnology progress·2024
Same author

23ME-00610, a genetically informed, first-in-class antibody targeting CD200R1 to enhance antitumor T cell function.

Oncoimmunology·2023

Related Experiment Video

Updated: Apr 4, 2026

Use of High-Throughput Automated Microbioreactor System for Production of Model IgG1 in CHO Cells
08:15

Use of High-Throughput Automated Microbioreactor System for Production of Model IgG1 in CHO Cells

Published on: September 28, 2018

11.6K

Application of high-throughput mini-bioreactor system for systematic scale-down modeling, process characterization,

Vijay Janakiraman1, Chris Kwiatkowski1, Rashmi Kshirsagar2

  • 1Cell Culture Development, Biogen, Inc., Research Triangle Park, NC, 27709.

Biotechnology Progress
|August 29, 2015
PubMed
Summary

High-throughput mini-bioreactors can replace traditional bench scale systems for bioprocess characterization. This advanced system accelerates studies, enabling faster development of control strategies for critical process parameters.

Keywords:
control strategymini-bioreactorsmultivariate analysisprocess characterizationscale-down modeling

More Related Videos

Process Optimization using High Throughput Automated Micro-Bioreactors in Chinese Hamster Ovary Cell Cultivation
09:28

Process Optimization using High Throughput Automated Micro-Bioreactors in Chinese Hamster Ovary Cell Cultivation

Published on: May 18, 2020

9.4K
Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation
12:04

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation

Published on: December 6, 2013

13.2K

Related Experiment Videos

Last Updated: Apr 4, 2026

Use of High-Throughput Automated Microbioreactor System for Production of Model IgG1 in CHO Cells
08:15

Use of High-Throughput Automated Microbioreactor System for Production of Model IgG1 in CHO Cells

Published on: September 28, 2018

11.6K
Process Optimization using High Throughput Automated Micro-Bioreactors in Chinese Hamster Ovary Cell Cultivation
09:28

Process Optimization using High Throughput Automated Micro-Bioreactors in Chinese Hamster Ovary Cell Cultivation

Published on: May 18, 2020

9.4K
Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation
12:04

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation

Published on: December 6, 2013

13.2K

Area of Science:

  • Biotechnology
  • Biopharmaceutical Process Development
  • Chemical Engineering

Background:

  • Traditional bioprocess characterization is time-consuming and resource-intensive.
  • Bench scale bioreactors are typically used for process characterization.
  • High-throughput systems are often limited to process development and optimization.

Purpose of the Study:

  • To evaluate the Advanced Microscale Bioreactor (ambr15™) system for bioprocess characterization.
  • To develop a scale-down model comparable to manufacturing and bench scales.
  • To demonstrate the system's capability for developing input control strategies.

Main Methods:

  • Development of a scale-down model in the ambr15™ system using statistical multivariate analysis.
  • Matching volumetric sparge rates and comparing pCO2 profiles with manufacturing scale.
  • Performing Design of Experiments (DoE) for process characterization and generating product quality data.
  • Comparing ambr15™ DoE results with bench scale bioreactor data.

Main Results:

  • The ambr15™ scale-down model demonstrated comparability with manufacturing (15,000 L) and bench (5 L) scales.
  • Process and product quality parameters were matched between ambr15™ and manufacturing scales.
  • Similar effects of process parameters on yield and quality were observed compared to bench scale studies.
  • Action limits for critical controlled parameters (CCPs) derived from ambr15™ data were comparable to bench scale results.

Conclusions:

  • The ambr15™ system can effectively replace bench scale bioreactors for routine process development and characterization.
  • This high-throughput system significantly reduces the time and resources required for process characterization.
  • The study validates the use of ambr15™ for developing robust input control strategies.