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Related Experiment Video

Updated: Apr 4, 2026

Two Methods of Heterokaryon Formation to Discover HCV Restriction Factors

Two Methods of Heterokaryon Formation to Discover HCV Restriction Factors

Published on: July 16, 2012

SRFBP1, an Additional Player in HCV Entry.

Lucie Fénéant¹, Laurence Cocquerel¹

¹Université Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 - UMR 8204 - CIIL -Centre d'Infection et d'Immunité de Lille, F-59000 Lille, France.

Trends in Microbiology

|August 31, 2015

Summary

Serum response factor binding protein 1 (SRFBP1) interacts with CD81 during hepatitis C virus (HCV) infection. This interaction is crucial for the post-fusion viral entry process, highlighting a new target for HCV therapies.

Keywords:

Hepatitis C Virus SRFBP1 entry factor protein-protein interactions proteomics the tetraspanin CD81

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Last Updated: Apr 4, 2026

Two Methods of Heterokaryon Formation to Discover HCV Restriction Factors

Two Methods of Heterokaryon Formation to Discover HCV Restriction Factors

Published on: July 16, 2012

A Protocol for Analyzing Hepatitis C Virus Replication

A Protocol for Analyzing Hepatitis C Virus Replication

Published on: June 26, 2014

Immunofluorescence to Monitor the Cellular Uptake of Human Lactoferrin and its Associated Antiviral Activity Against the Hepatitis C Virus

Immunofluorescence to Monitor the Cellular Uptake of Human Lactoferrin and its Associated Antiviral Activity Against the Hepatitis C Virus

Published on: October 1, 2015

Area of Science:

Virology
Molecular Cell Biology
Immunology

Background:

Tetraspanin CD81 is vital for hepatitis C virus (HCV) entry into host cells.
Protein interactions involving CD81 regulate viral pathogenesis.
Understanding CD81's partners is key to developing antiviral strategies.

Purpose of the Study:

To identify novel proteins interacting with CD81 during HCV infection.
To elucidate the role of these interactions in the viral post-fusion entry stage.

Main Methods:

Proteomic analysis was employed to identify CD81-interacting proteins.
Co-immunoprecipitation assays confirmed protein-protein interactions.
HCV infection models were used to study the functional relevance of identified proteins.

Main Results:

Serum response factor binding protein 1 (SRFBP1) was identified as a novel CD81-interacting protein.
SRFBP1 was found to associate with CD81 during the post-fusion phase of HCV entry.
This interaction plays a role in facilitating viral entry after membrane fusion.

Conclusions:

SRFBP1 is a key mediator in the CD81-dependent post-fusion entry of HCV.
Targeting the SRFBP1-CD81 interaction may offer a new therapeutic strategy against HCV infection.