[Electrokinetic potential of cell nucleus during changes in the functional activity of rat hepatocytes]

There were investigated changes of electrokinetic potential (EKP) of hepatocyte nuclei during hydrocortisone induction and activation induced with amino acids mixture. An increase of RNA-polymerase activity was shown to correlate with EKP value. Actinomycin D essentially decreased this potential. Injection of hydrocortisone or amino acids mixture under the suppression of transcription with actinomycin D removed the inhibitor action, but the potential value did not reach that under the action of the activators only. Induction with hydrocortisone increased the potential by various degrees for the nuclei of different size. The results obtained suggest that EKP of the nuclei carries the information about functional condition of the genetic apparatus and can be a valuable and handy instrument of definition of its physiological activity.