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Published on: October 12, 2017
[ANTIOXIDANT DYSFUNCTIONALITY OF HIGH-DENSITY LIPOPROTEINS (HDL) IN DECOMPENSATED DIABETIC PATIENTS]
Fernanda Awad1, Susana Contreras-Duarte2, Patricia Molina3
1Departamento de Nutrición, Diabetes y Metabolismo. Pontificia Universidad Católica de Chile, Santiago. (Chile).. ferawadm@gmail.com.
Insights
High-density lipoproteins (HDL) lose antioxidant function during diabetic crises. This function recovers with treatment, likely due to restored paraoxonase-1 activity, highlighting HDL’s role in diabetes management.
Area of Science:
- Cardiovascular Science
- Metabolic Disorders
- Lipid Metabolism
Background:
- High-density lipoproteins (HDL) possess crucial cardiovascular protective functions, including antioxidant and anti-inflammatory properties.
- HDL's protective functions are known to be impaired in stable diabetes.
- However, HDL's antioxidant capacity during acute hyperglycemic crises remains unevaluated.
Purpose of the Study:
- To assess HDL's antioxidant activity during severe diabetic decompensation.
- To investigate the restoration of HDL antioxidant function after the acute event resolves.
Main Methods:
- HDL antioxidant activity was quantified using an in vitro fluorescent assay.
- Plasma samples were collected from diabetic patients during acute decompensation, hospital recovery, and ambulatory follow-up.
- HDL from healthy individuals served as controls.
Main Results:
- HDL antioxidant function was significantly diminished in patients with acute diabetic decompensation compared to controls.
- A gradual restoration of HDL antioxidant function was observed during ambulatory follow-up, reaching normal levels.
- Low plasma paraoxonase-1 activity during hyperglycemic crisis increased significantly at follow-up.
Conclusions:
- HDL particles from patients in acute diabetic decompensation exhibit significantly reduced and reversible antioxidant capacity.
- This reduction in antioxidant capacity is likely attributed to decreased paraoxonase-1 activity.
- The findings suggest a transient impairment of HDL antioxidant function during diabetic crises, which is recoverable.
Introduction:
high density lipoproteins (HDL) have important cardiovascular protective effects mediated by their role in reverse cholesterol transport as well as other functional activities, including significant anti-inflammatory and antioxidant properties. It has been shown that HDL anti-inflammatory and antioxidant functions are defective in metabolically stable diabetic patients; however they have not been evaluated during a hyperglycemic crisis.
Aim:
to determine the antioxidant activity of HDL during a severe diabetic decompensation and to analyze whether this function is restored after resolution of the acute event.
Methods:
the antioxidant activity of HDL was measured in vitro by a fluorescent assay in plasma samples obtained from diabetic patients with acute metabolic decompensation at admission, recovery within the hospital and follow-up in ambulatory care. As a comparison, HDL particles from some healthy subjects were used as controls.
Results:
the HDL antioxidant function was significantly reduced in patients during an acute diabetic decompensation compared with the control group, and was gradually restored reaching normal values during the ambulatory follow-up. Hyperglycemic crisis also showed low plasma paraoxonase-1 activity, which increased significantly during at follow-up.
Conclusion:
HDL particles isolated from acute diabetic descompensated patients exhibit a significantly and reversibly low antioxidant capacity, which is probably due to a reduced paraoxonase-1 activity.
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