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Updated: Apr 4, 2026
Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen
Published on: June 3, 2018
Using the synergism strategy for highly sensitive and specific electrochemical sensing of Streptococcus pneumoniae
Fengqin Li1, Zhigang Yu1, Yanmei Xu1
1College of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin 150080, China.
Abstract:
With the help of the interaction mode of capture probe-target-signal probe (CP-T-SP), an electrochemical sensing method based on the synergism strategy of dual-hybridized signaling probes modified with 6 MB (methylene blue), background suppression and large surface area Au electrode is developed for the detection of Streptococcus pneumoniae (S. pneumoniae) Lyt-1 gene sequence. The proposed sensor features a very low detection limit (LOD) of ∼0.5 fM for the target. This method also exhibits highly versatility and can apply to the construction of other sensors for the analysis of similar designated pathogenic bacteria gene sequence (PBGS).
