Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

8.6K
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
8.6K
Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

1.9K
1.9K
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

11.0K
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
11.0K
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

2.6K
2.6K
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

2.6K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.6K
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

4.0K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
4.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nutrient uptake and metabolism in osteoblasts.

Current opinion in endocrine and metabolic research·2026
Same author

TGFβ activity stabilizes ACC1 to increase de novo lipogenesis in metabolic liver disease.

Molecular metabolism·2026
Same author

Pulsatile flow dynamics maintain pulmonary artery architecture.

JCI insight·2026
Same author

Psychiatric Morbidity After Digital Replantation Versus Amputation.

Hand (New York, N.Y.)·2026
Same author

Fibrin defines tissue stiffness and biomechanical signaling in regenerating zebrafish hearts as revealed by high-resolution stiffness mapping.

iScience·2026
Same author

PRINCIPLES GOVERNING ENDOTHELIAL CAVEOLAE ORGANIZATION DURING ANGIOGENESIS.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Apr 4, 2026

Studying Wnt Signaling During Patterning of Conducting Airways
13:00

Studying Wnt Signaling During Patterning of Conducting Airways

Published on: October 16, 2016

7.9K

Wnt4 is essential to normal mammalian lung development.

Arianna Caprioli1, Alethia Villasenor2, Lyndsay A Wylie3

  • 1Dept. of Biology and Physical Sciences, Marymount Univ., 2807 N. Glebe Rd., Arlington, VA 22207, USA.

Developmental Biology
|September 1, 2015
PubMed
Summary

Wingless-related integration (Wnt) protein 4 (Wnt4) is crucial for mammalian respiratory system development. Wnt4 deficiency in mice leads to lung hypoplasia and tracheal defects, highlighting its essential role in lung organogenesis.

Keywords:
Cell proliferationFgf10Fgf9Lung developmentTracheaTtf1Wnt2Wnt4

More Related Videos

Mouse Embryonic Lung Culture, A System to Evaluate the Molecular Mechanisms of Branching
07:32

Mouse Embryonic Lung Culture, A System to Evaluate the Molecular Mechanisms of Branching

Published on: June 30, 2010

19.1K
The Soft Agar Colony Formation Assay
08:01

The Soft Agar Colony Formation Assay

Published on: October 27, 2014

114.4K

Related Experiment Videos

Last Updated: Apr 4, 2026

Studying Wnt Signaling During Patterning of Conducting Airways
13:00

Studying Wnt Signaling During Patterning of Conducting Airways

Published on: October 16, 2016

7.9K
Mouse Embryonic Lung Culture, A System to Evaluate the Molecular Mechanisms of Branching
07:32

Mouse Embryonic Lung Culture, A System to Evaluate the Molecular Mechanisms of Branching

Published on: June 30, 2010

19.1K
The Soft Agar Colony Formation Assay
08:01

The Soft Agar Colony Formation Assay

Published on: October 27, 2014

114.4K

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • Wnt signaling pathways are vital for embryonic development and organogenesis.
  • Wnt4 is a key Wnt family member implicated in the development of various organs, including kidneys and mammary glands.

Purpose of the Study:

  • To investigate the role of Wnt4 in mammalian respiratory system development.
  • To identify novel expression patterns of Wnt4 during lung organogenesis.

Main Methods:

  • In situ hybridization in mouse embryos to detect Wnt4 expression.
  • Analysis of Wnt4 knockout (Wnt4-/-) mouse embryos to assess lung and tracheal development.
  • Assessment of cell proliferation and expression of key lung growth factors (Fgf9, Fgf10, Sox9, Wnt2) in Wnt4-/- embryos.

Main Results:

  • Wnt4 is expressed in the anterior trunk mesoderm adjacent to the tracheoesophageal endoderm during early embryonic development.
  • Wnt4-/- embryos exhibit significant lung hypoplasia and tracheal abnormalities.
  • Reduced cell proliferation was observed in Wnt4-/- lung buds, particularly in tip domains.
  • Expression of crucial lung development factors (Fgf9, Fgf10, Sox9, Wnt2) and the progenitor factor Sox9 in the trachea was decreased in Wnt4-/- embryos.

Conclusions:

  • Wnt4 plays a critical, previously unrecognized role in the proper morphogenesis and growth of the mammalian respiratory system.
  • Wnt4 influences lung development by regulating cell proliferation and the expression of essential growth factors and progenitor factors.