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Centrifugal loop-mediated isothermal amplification microdevice for rapid, multiplex and colorimetric foodborne
Seung Jun Oh1, Byung Hyun Park1, Jae Hwan Jung1
1Department of Chemical and Biomolecular Engineering (BK21 PLUS Program), Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Biosensors & Bioelectronics
|September 1, 2015
Summary
This study introduces a microfluidic device for rapid, multiplex foodborne pathogen identification using loop-mediated isothermal amplification (LAMP). The system achieves sensitive detection of bacteria like E. coli O157:H7 in under an hour.
Area of Science:
- Biotechnology
- Microfluidics
- Food Safety
Background:
- Foodborne pathogens pose significant public health risks.
- Current detection methods can be time-consuming and require specialized equipment.
- Multiplex detection of multiple pathogens simultaneously is desirable.
Purpose of the Study:
- To develop a centrifugal microfluidic device for rapid, high-throughput identification of foodborne pathogens.
- To integrate loop-mediated isothermal amplification (LAMP) and colorimetric detection for simplified analysis.
- To enable point-of-care (POC) testing for food safety applications.
Main Methods:
- A centrifugal microfluidic system with five identical structures was designed for genetic analysis.
- Optimized zigzag microchannels and rotational speed (RPM) controlled sequential reagent and sample loading.
- Loop-mediated isothermal amplification (LAMP) targeted specific pathogenic bacteria, with results detected colorimetrically using Eriochrome Black T (EBT).
Main Results:
- The device enabled multiplex identification of Escherichia coli O157:H7, Salmonella typhimurium, and Vibrio parahaemolyticus.
- A limit of detection (LOD) of 380 copies for E. coli O157:H7 was achieved.
- Specific primer sets allowed selective and sensitive on-chip identification, visible to the naked eye.
- The entire process, from sample to result, was completed within 60 minutes.
Conclusions:
- The developed centrifugal microfluidic device offers a simple, rapid, and sensitive platform for foodborne pathogen detection.
- The system's ability to perform multiplex analysis without bulky instrumentation makes it suitable for point-of-care (POC) applications.
- This microsystem represents an advancement in genetic analysis for enhanced food safety monitoring.
