Related Experiment Videos

Mobilization of murine hemopoietic stem cells (HSC) by Pyran Copolymer

A R Zander, K A Dicke, D Verma

    Experimental Hematology
    |January 1, 1979
    PubMed

    Insights

    Pyran Copolymer significantly boosts circulating stem cells in mice. This polymer, divinyl-ether maleic anhydride, mobilizes hemopoietic stem cells effectively, with effects dependent on molecular weight.

    Area of Science:

    • Hematology
    • Polymer Science

    Background:

    • Pluripotential stem cells are crucial for blood cell production.
    • Mobilization of stem cells is a key process in hematopoiesis.

    Purpose of the Study:

    • To investigate the effect of Pyran Copolymer (divinyl-ether maleic anhydride) on circulating stem cell concentrations.
    • To determine the relationship between Pyran Copolymer molecular weight and stem cell mobilization.

    Main Methods:

    • Administration of Pyran Copolymer to mice.
    • Quantification of circulating colony-forming unit-spleen (CFU-S) and colony-forming unit-culture (CFU-C).
    • Analysis of stem cell mobilization across a range of Pyran Copolymer molecular weights (12,000–52,000).

    Main Results:

    • Pyran Copolymer increased circulating pluripotential stem cells 15- to 30-fold.
    • Maximal mobilization occurred five days post-injection, with synchronous peaks in CFU-S and CFU-C.
    • Stem cell mobilization correlated positively with Pyran Copolymer molecular weight.

    Conclusions:

    • Divinyl-ether maleic anhydride is a potent agent for mobilizing hemopoietic stem cells.
    • The molecular weight of Pyran Copolymer influences its efficacy in stem cell mobilization.
    • Mobilized stem cells migrate from bone marrow to peripheral blood and are sequestered in the spleen.

    Related Concept Videos