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Updated: Apr 4, 2026

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Published on: June 1, 2019
Ultrasound-guided percutaneous injection of methylene blue to identify nerve pathology and guide surgery
Joseph A Osorio1, Jonathan D Breshears1, Omar Arnaout2
1Department of Neurological Surgery, University of California, San Francisco, California;
Abstract:
OBJECT The objective of this study was to provide a technique that could be used in the preoperative period to facilitate the surgical exploration of peripheral nerve pathology. METHODS The authors describe a technique in which 1) ultrasonography is used in the immediate preoperative period to identify target peripheral nerves, 2) an ultrasound-guided needle electrode is used to stimulate peripheral nerves to confirm their position, and then 3) a methylene blue (MB) injection is performed to mark the peripheral nerve pathology to facilitate surgical exploration. RESULTS A cohort of 13 patients with varying indications for peripheral nerve surgery is presented in which ultrasound guidance, stimulation, and MB were used to localize and create a road map for surgeries. CONCLUSIONS Preoperative ultrasound-guided MB administration is a promising technique that peripheral nerve surgeons could use to plan and execute surgery.
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