Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Carbon-dioxide Fixation01:28

Carbon-dioxide Fixation

874
Carbon dioxide fixation in prokaryotes enables the assimilation of inorganic carbon into organic molecules, supporting biosynthetic pathways, sustaining ecosystems, and contributing to the global carbon cycle. It also has industrial applications in carbon capture and bioproduct synthesis. Autotrophic organisms rely on this process to utilize CO₂ as a carbon source in diverse environments.The Calvin CycleThe Calvin cycle is the most widespread carbon fixation mechanism, primarily used by...
874
The Calvin Benson Cycle01:46

The Calvin Benson Cycle

7.8K
Ribulose 1,5- bisphosphate carboxylase/oxygenase (RuBisCo) is a critical enzyme that catalyzes carbon dioxide assimilation during photosynthesis. However, it is an inefficient enzyme, having an extremely slow catalytic rate. A typical enzyme can process about a thousand molecules per second; however, RuBisCo fixes only around three-carbon dioxides per second. Photosynthetic cells compensate for this slow rate by synthesizing very high amounts of RuBisCo, making it the most abundant single...
7.8K
C4 Pathway and CAM01:27

C4 Pathway and CAM

50.7K
Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...
50.7K
Bioreactor Controls-I01:28

Bioreactor Controls-I

64
Maintaining optimal conditions within fermenters is essential for maximizing microbial productivity and ensuring process efficiency. This lesson focuses on key parameters—temperature, foam, pH, carbon dioxide, oxygen, and pressure—and their precise measurement and control strategies in fermentation systems.Temperature ControlTemperature regulation is critical due to the exothermic nature of many fermentation processes. In small laboratory fermenters, temperature is commonly...
64
Bioreactor Controls-II01:18

Bioreactor Controls-II

56
In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the...
56
Bioremediation00:46

Bioremediation

23.0K
Bioremediation is the use of prokaryotes, fungi, or plants to remove pollutants from the environment. This process has been used to remove harmful toxins in groundwater as a byproduct of agricultural run-off and also to clean up oil spills.
23.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

CRISPRi screening identifies SON and MAP4K1 as regulators of type III cytokine expression in innate lymphoid cells.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same author

High-resolution promoter interaction analysis implicates genes involved in the activation of Type 3 Innate Lymphoid Cells in autoimmune disease risk.

bioRxiv : the preprint server for biology·2026
Same author

<i>Escherichia coli</i> is poised to grow using 5'-deoxynucleosides via MtnR and CRP regulation of DHAP shunt gene expression.

Journal of bacteriology·2025
Same author

CRISPRi Screening Identifies SON and MAP4K1 as Regulators of Type III Cytokine Expression in Innate Lymphoid Cells.

bioRxiv : the preprint server for biology·2025
Same author

<i>Escherichia coli</i> possessing the dihydroxyacetone phosphate shunt utilize 5'-deoxynucleosides for growth.

Microbiology spectrum·2024
Same author

Utilization of 5'-deoxy-nucleosides as Growth Substrates by Extraintestinal Pathogenic <i>E. coli</i> via the Dihydroxyacetone Phosphate Shunt.

bioRxiv : the preprint server for biology·2023

Related Experiment Video

Updated: Apr 4, 2026

Establishment of Microbial Eukaryotic Enrichment Cultures from a Chemically Stratified Antarctic Lake and Assessment of Carbon Fixation Potential
14:38

Establishment of Microbial Eukaryotic Enrichment Cultures from a Chemically Stratified Antarctic Lake and Assessment of Carbon Fixation Potential

Published on: April 20, 2012

11.9K

CbbR, the Master Regulator for Microbial Carbon Dioxide Fixation.

Andrew W Dangel1, F Robert Tabita2

  • 1Department of Microbiology, The Ohio State University, Columbus, Ohio, USA.

Journal of Bacteriology
|September 2, 2015
PubMed
Summary
This summary is machine-generated.

Biological carbon dioxide fixation, essential for life, relies on the Calvin-Bassham-Benson pathway. The regulator CbbR controls this process, with its activity optimized through various interactions for efficient carbon conversion.

More Related Videos

Operation of Laboratory Photobioreactors with Online Growth Measurements and Customizable Light Regimes
05:21

Operation of Laboratory Photobioreactors with Online Growth Measurements and Customizable Light Regimes

Published on: October 28, 2021

2.7K
High-Throughput Metabolic Profiling for Model Refinements of Microalgae
11:07

High-Throughput Metabolic Profiling for Model Refinements of Microalgae

Published on: December 4, 2021

4.4K

Related Experiment Videos

Last Updated: Apr 4, 2026

Establishment of Microbial Eukaryotic Enrichment Cultures from a Chemically Stratified Antarctic Lake and Assessment of Carbon Fixation Potential
14:38

Establishment of Microbial Eukaryotic Enrichment Cultures from a Chemically Stratified Antarctic Lake and Assessment of Carbon Fixation Potential

Published on: April 20, 2012

11.9K
Operation of Laboratory Photobioreactors with Online Growth Measurements and Customizable Light Regimes
05:21

Operation of Laboratory Photobioreactors with Online Growth Measurements and Customizable Light Regimes

Published on: October 28, 2021

2.7K
High-Throughput Metabolic Profiling for Model Refinements of Microalgae
11:07

High-Throughput Metabolic Profiling for Model Refinements of Microalgae

Published on: December 4, 2021

4.4K

Area of Science:

  • Microbiology
  • Biochemistry
  • Molecular Biology

Background:

  • Biological carbon dioxide fixation is vital for converting atmospheric CO2 into organic carbon.
  • The Calvin-Bassham-Benson (CBB) pathway, particularly the RubisCO enzyme, is central to this process.
  • Gene expression for the CBB pathway is regulated by the transcriptional regulator CbbR.

Purpose of the Study:

  • To explore the regulatory mechanisms of CbbR in controlling carbon dioxide fixation pathways.
  • To understand how CbbR interacts with metabolites and other regulators to optimize gene expression.
  • To identify strategies for enhancing microbial carbon fixation for bioproduct applications.

Main Methods:

  • Analysis of CbbR protein family and their interactions.
  • Investigating the role of effector metabolites and post-translational modifications on CbbR activity.
  • Examining the impact of different CbbR variants on cbb gene expression.

Main Results:

  • CbbR proteins exhibit versatility in binding various effector metabolites, influencing their transcriptional control.
  • Post-translational modifications of CbbR by two-component systems further refine cbb operon expression.
  • Constitutively active CbbR variants can enhance cbb gene expression levels.

Conclusions:

  • CbbR-dependent regulation is a key factor in optimizing biological carbon dioxide fixation.
  • Understanding these regulatory networks is crucial for harnessing microbial capabilities for CO2 conversion.
  • Further research into CbbR optimization holds potential for developing biotechnological applications.