Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Amplifying Signals via Enzymatic Cascade01:22

Amplifying Signals via Enzymatic Cascade

19.5K
When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze...
19.5K
Enzyme-linked Receptors01:00

Enzyme-linked Receptors

89.1K
Enzyme-linked receptors are proteins that act as both receptor and enzyme, activating multiple intracellular signals. This is a large group of receptors that include the receptor tyrosine kinase (RTK) family. Many growth factors and hormones bind to and activate the RTKs.
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
89.1K
PCR01:32

PCR

242.1K
Overview
242.1K
Real Time RT-PCR02:57

Real Time RT-PCR

66.8K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
66.8K
Amplifying Signals via Second Messengers01:15

Amplifying Signals via Second Messengers

9.5K
Many receptor binding ligands are hydrophilic; they do not cross the cell membrane but bind to cell-surface receptors. Thus, their message must be relayed by second messengers present in the cell cytoplasm. There are several second messenger pathways, each with its own way of relaying information. For example, the G protein-coupled receptors can activate both phosphoinositol and cyclic AMP (cAMP) second messenger pathways. The phosphoinositol pathway is active when the receptor induces...
9.5K
Transducer Mechanism: Enzyme-Linked Receptors01:27

Transducer Mechanism: Enzyme-Linked Receptors

4.9K
Enzyme-linked receptors are cell-surface receptors acting as an enzyme or associating with an enzyme intracellularly. They make excellent drug targets. Drugs can bind to the extracellular ligand-binding domain or directly affect their enzymatic domain and alter their activity.
Major types that are helpful drug targets include:
4.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Dissipative Reaction Network Drives Transient Solid-Liquid and Liquid-Liquid Phase Cycling of Nanoparticles.

Angewandte Chemie (International ed. in English)·2023
Same author

An electric molecular motor.

Nature·2023
Same author

A Molecular Replication Process Drives Supramolecular Polymerization.

Journal of the American Chemical Society·2021
Same author

Encoding Multiple Reactivity Modes within a Single Synthetic Replicator.

Journal of the American Chemical Society·2020
Same author

Single-Crystal Polycationic Polymers Obtained by Single-Crystal-to-Single-Crystal Photopolymerization.

Journal of the American Chemical Society·2020
Same author

Combining Intra- and Intermolecular Charge Transfer with Polycationic Cyclophanes To Design 2D Tessellations.

Journal of the American Chemical Society·2019
Same journal

Diversity of dye aggregates: self-assembly of naphthalene-linked chlorophyll dyads dependent on substitution position.

Organic & biomolecular chemistry·2026
Same journal

Copper-catalyzed C-N cross-coupling of (hetero)aryl bromides with <i>N</i>-Boc hydrazine.

Organic & biomolecular chemistry·2026
Same journal

<i>In situ</i> generated iridium nanoparticle-catalyzed highly efficient hydrogenation of nitroarenes.

Organic & biomolecular chemistry·2026
Same journal

Azo compounds as key intermediates in the synthesis of cinnolines. Recent advances.

Organic & biomolecular chemistry·2026
Same journal

Cyclopentannulation toward hydrindane frameworks for the tricyclic core of (±)-rumphellclovane E and (±)-sarinfacetamides A and B.

Organic & biomolecular chemistry·2026
Same journal

Rosamine derivatives of <i>o</i>-aminothiophenol-triacetate (S-APTRA): a new class of selective fluorescent sensors for Zn<sup>2</sup>.

Organic & biomolecular chemistry·2026
See all related articles

Related Experiment Video

Updated: Apr 4, 2026

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
03:38

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

2.0K

A recognition-mediated reaction drives amplification within a dynamic library.

Jan W Sadownik1, Douglas Philp

  • 1School of Chemistry and EaStCHEM, University of St Andrews, North Haugh, St Andrews, Fife KY16 9ST, UK. d.philp@st-andrews.ac.uk.

Organic & Biomolecular Chemistry
|September 2, 2015
PubMed
Summary
This summary is machine-generated.

A novel recognition event precisely modifies dynamic combinatorial libraries. This targeted cycloaddition reaction alters the library's composition and kinetic behavior.

More Related Videos

Linear Amplification Mediated PCR &#8211; Localization of Genetic Elements and Characterization of Unknown Flanking DNA
11:58

Linear Amplification Mediated PCR – Localization of Genetic Elements and Characterization of Unknown Flanking DNA

Published on: June 25, 2014

31.0K
Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
07:55

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

Published on: September 11, 2022

2.4K

Related Experiment Videos

Last Updated: Apr 4, 2026

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
03:38

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

2.0K
Linear Amplification Mediated PCR &#8211; Localization of Genetic Elements and Characterization of Unknown Flanking DNA
11:58

Linear Amplification Mediated PCR – Localization of Genetic Elements and Characterization of Unknown Flanking DNA

Published on: June 25, 2014

31.0K
Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
07:55

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

Published on: September 11, 2022

2.4K

Area of Science:

  • Chemistry
  • Chemical Biology
  • Molecular Recognition

Background:

  • Dynamic combinatorial libraries (DCLs) are powerful tools for discovering new molecular entities.
  • Controlling the composition and behavior of DCLs remains a significant challenge.

Purpose of the Study:

  • To develop a method for precisely controlling the composition and kinetic behavior of DCLs.
  • To demonstrate the utility of recognition-mediated reactions in DCL manipulation.

Main Methods:

  • Design of a specific recognition event to target one component of a DCL.
  • Implementation of an irreversible cycloaddition reaction triggered by the recognition event.
  • Analysis of the DCL composition and kinetics before and after the recognition event.

Main Results:

  • A single recognition event successfully and selectively transformed one reactive member of the DCL.
  • The cycloaddition reaction irreversibly altered the DCL's composition.
  • The kinetic behavior of the DCL was dramatically changed due to the selective transformation.

Conclusions:

  • Recognition-mediated irreversible reactions offer a powerful strategy for controlling DCLs.
  • This approach enables precise manipulation of molecular libraries for targeted discovery.
  • The findings open new avenues for designing sophisticated molecular systems.