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Published on: May 26, 2017
MAP kinase phosphatase 3 inhibits brown adipocyte differentiation via regulation of Erk phosphorylation
Won Kon Kim1, Kyoung-Jin Oh2, Hye-Ryung Choi2
1Functional Genomics Research Center, KRIBB, Daejeon, 305-806, Republic of Korea; Department of Functional Genomics, University of Science and Technology of Korea, Daejeon, 305-806, Republic of Korea.
Abstract:
Brown fat has been highlight as a new therapeutic target for treatment of obesity and diabetes. However, molecular mechanism underlying brown adipogenesis are not fully understood. Here, we identified that MAP kinase phosphatase 3 (MKP3) has a novel role as regulator of brown adipocyte differentiation. The expression of MKP3 was significantly decreased during the early stage(s) of brown adipocyte differentiation in HIB-1B cells and primary cells. Ectopic expression of MKP3 led to reduced brown adipocyte differentiation, whereas depletion of MKP3 significantly enhanced the differentiation of primary brown preadipocytes. Consistently, we found an increased brown adipocyte differentiation in MKP3-null MEF cells. These inhibitory effects of MKP3 could be resulted via the temporal regulation of Erk activation. In recent, it was reported that MKP3 deficient mice are resistant to diet-induced obesity, and display enhanced energy expenditure. Taken together, we suggest that MKP3 could be an important factor in the regulation of brown adipocyte differentiation.
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