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Dipolar rearrangement during micellization explored using a potential-sensitive fluorescent probe.

Parijat Sarkar¹, Amitabha Chattopadhyay¹

¹CSIR-Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India.

Chemistry and Physics of Lipids

|September 2, 2015

Summary

Micelle formation involves dipolar rearrangement, altering dipole potential. This study demonstrates a new method using this change to measure critical micelle concentration (CMC) for detergents.

Keywords:

CMC Detergents Dipolar reorganization Dipole potential Micelles di-8-ANEPPS

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Area of Science:

Physical chemistry
Biophysics
Surface chemistry

Background:

Dipole potential arises from the ordered arrangement of molecules at interfaces.
While studied in lipid bilayers, dipole potential's role in other molecular assemblies like micelles is less understood.

Purpose of the Study:

To investigate dipolar rearrangement during micelle formation.
To explore the use of dipole potential changes for determining critical micelle concentration (CMC).

Main Methods:

Utilized a dual wavelength ratiometric approach with the potential-sensitive probe di-8-ANEPPS.
Monitored changes in dipole potential during detergent micellization.

Main Results:

Observed and quantified dipolar reorganization accompanying micelle formation.
Successfully estimated CMC values for various detergents, showing good agreement with literature data.

Conclusions:

Micelle formation is intrinsically linked to dipolar reorientation.
Dipole potential measurement offers a novel technique for characterizing micellar organization and determining CMC.