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Updated: Apr 4, 2026
Surgical Models of Gastroesophageal Reflux with Mice
Published on: August 25, 2015
Surgical Models of Gastroesophageal Reflux with Mice
Jinxi He1, Yu Fang2, Xiaoxin Chen3
1Department of Thoracic Surgery, Ningxia Medical University General Hospital; Cancer Research Program, North Carolina Central University.
Abstract:
Multiple surgical procedures have been reported to induce gastroesophageal reflux in animals. Herein, we report three surgical models with mice aiming to induce reflux of gastric contents, duodenal contents or mixed contents. Surgical procedures and general principles have been described in detail. A researcher with surgical experience should be able to grasp the technique after a short period of practice. After surgery, most mice can survive and develop reflux esophagitis similar to that in humans. However, it should be noted that histological differences between mouse and human esophagus are the inherent limitations of these surgical models. If used for research on Barrett's esophagus and adenocarcinoma, these procedures may need to be combined with genetic modifications.
Insights
Researchers developed three mouse surgical models to induce gastroesophageal reflux, mimicking human conditions for studying reflux esophagitis. These models offer a valuable tool for preclinical research, though histological differences necessitate caution for specific applications.
Area of Science:
- Gastroenterology
- Surgical Pathology
- Animal Models
Background:
- Gastroesophageal reflux disease (GERD) affects millions globally, necessitating robust animal models for research.
- Previous surgical methods for inducing reflux in animals have limitations.
- Developing reliable models is crucial for understanding GERD pathogenesis and testing therapies.
Purpose of the Study:
- To establish and describe three distinct surgical models in mice for inducing gastroesophageal reflux.
- To enable the study of refluxate from gastric, duodenal, or mixed sources.
- To provide a foundation for research into reflux-induced esophageal conditions.
Main Methods:
- Detailed description of three surgical procedures in mice designed to induce reflux.
- Focus on surgical techniques and general principles for reproducibility.
- Post-operative care and monitoring protocols for animal survival and model development.
Main Results:
- Successful induction of reflux in mouse models, leading to conditions resembling human reflux esophagitis.
- High survival rates observed in post-operative mice.
- Demonstration of the ability to induce reflux of gastric, duodenal, or mixed contents.
Conclusions:
- The described surgical models provide a viable platform for studying reflux esophagitis in mice.
- These models can be adapted for research into Barrett's esophagus and adenocarcinoma, potentially with genetic modifications.
- Surgical expertise is recommended for successful implementation and interpretation of results.
