Related Experiment Video
Updated: Apr 4, 2026
10:23
Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
Published on: April 17, 2017
10.8K
STAT3‑regulated long non‑coding RNAs lnc‑7SK and lnc‑IGF2‑AS promote hepatitis C virus replication
Yulin Xiong1, Ming Jia1, Jing Yuan1
1Key Laboratory of Infectious Disease Research, Institute of Infectious Diseases of Chinese PLA, Southwest Hospital, Third Military Medical University, Chongqing 400038, P.R. China.
Molecular Medicine Reports
|September 3, 2015
Summary
This study identifies two long non-coding RNAs (lncRNAs), lnc-IGF2-AS and lnc-7SK, that are upregulated by STAT3 and influence hepatitis C virus (HCV) replication by regulating phosphatidylinositol 4-phosphate (PI4P). These findings offer new insights into HCV infection mechanisms.
Area of Science:
- Molecular Biology
- Virology
- Genetics
Background:
- Long non-coding RNAs (lncRNAs) play crucial roles in biological processes.
- While many lncRNAs are linked to viral infections, their role in hepatitis C virus (HCV) replication is underexplored.
- Signal transducer and activator of transcription 3 (STAT3) is activated by HCV and promotes viral replication, but the underlying molecular mechanisms require further elucidation.
Purpose of the Study:
- To identify lncRNAs regulated by STAT3 that are involved in HCV replication.
- To investigate the role of identified lncRNAs in regulating HCV replication and associated molecular pathways.
Main Methods:
- Utilized human lncRNA polymerase chain reaction (PCR) arrays to screen for STAT3-upregulated lncRNAs.
- Employed small interfering RNA (siRNA) to transfect and inhibit specific lncRNAs (lnc-IGF2-AS and lnc-7SK) in HCV-infected Huh7 cells.
- Quantified changes in HCV replication and phosphatidylinositol 4-phosphate (PI4P) expression.
Main Results:
- Identified four lncRNAs (lnc-IGF2-AS, lnc-7SK, lnc-SChLAP1, and lnc-SRA1) upregulated by STAT3.
- Confirmed that lnc-IGF2-AS and lnc-7SK are involved in HCV replication.
- Demonstrated that siRNA-mediated inhibition of lnc-IGF2-AS and lnc-7SK partially reduced HCV replication.
- Showed that inhibiting these lncRNAs also decreased the expression of PI4P, a key factor in HCV replication.
Conclusions:
- lnc-IGF2-AS and lnc-7SK are novel lncRNAs upregulated by STAT3 during HCV infection.
- These lncRNAs contribute to HCV replication, potentially through the regulation of PI4P expression.
- Targeting lnc-IGF2-AS and lnc-7SK may represent a potential therapeutic strategy for hepatitis C virus infection.
Related Concept Videos
02:39lncRNA - Long Non-coding RNAs
10.2K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
10.2K
02:39lncRNA - Long Non-coding RNAs
3.9K
3.9K
01:30Inhibitors of Viral Protein Synthesis
32
Protein synthesis is indispensable for viral replication, as viruses lack the cellular machinery required for this process and must hijack the host's translational apparatus. In response, host cells deploy a critical innate immune defense involving interferons, specialized cytokines that play a central role in inhibiting viral propagation.Upon viral detection, infected cells release interferons that bind to receptors on adjacent uninfected cells, activating the JAK-STAT signaling pathway and...
32
01:25Hepatitis
42
Hepatitis is an inflammatory condition of the liver most commonly caused by hepatotropic viruses (A–E), though non-infectious causes such as alcohol and drugs also exist.Hepatitis AHepatitis A virus (HAV) is a non-enveloped RNA virus of the Picornaviridae family. It is primarily transmitted via the fecal-oral route, typically through ingestion of contaminated food or water. After ingestion, HAV enters the bloodstream through the oropharynx or intestinal epithelium and reaches the liver.
42
02:30siRNA - Small Interfering RNAs
19.0K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
19.0K
01:29Viruses with RNA Genomes
1.3K
RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
1.3K