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Enzyme-Linked Immunosorbent Assay

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Updated: Apr 4, 2026

Dual-modality Molecular Cartography: Integrating Multiplex mRNA Detection with Protein Imaging Mass Cytometry

Dual-modality Molecular Cartography: Integrating Multiplex mRNA Detection with Protein Imaging Mass Cytometry

Published on: November 14, 2025

Multiplexed In-cell Immunoassay for Same-sample Protein Expression Profiling.

Jing Shang¹, Pavel Zrazhevskiy¹, Nadia Postupna²

¹Department of Bioengineering, University of Washington, Seattle, WA 98195, USA.

Scientific Reports

|September 3, 2015

Summary

This summary is machine-generated.

This study introduces a novel multiplexed in-cell immunoassay for simultaneous protein expression analysis. This method accurately quantifies multiple protein targets in single specimens, overcoming limitations of existing techniques for biomedical research.

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High-Throughput Automated Multiplex Immunofluorescence Assays for Translational Research

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Published on: June 10, 2025

Area of Science:

Biochemistry
Molecular Biology
Cell Biology

Background:

In-cell immunoassays are crucial for protein expression analysis.
Current methods face challenges in multiplexing and technical complexity.
A need exists for robust, simultaneous protein profiling in single specimens.

Purpose of the Study:

To develop a simple and robust methodology for multiplexed protein expression profiling on intact specimens.
To overcome limitations of singleplex reporter enzymes in traditional immunoassays.
To enable accurate quantification of multiple protein targets within the same sample.

Main Methods:

Utilized alkaline phosphatase as a reporter enzyme for quantification.
Implemented a DNA-programmed release mechanism for reporter segregation.
Demonstrated proof-of-principle using cultured HeLa cells and human brain tissue sections.

Main Results:

Successfully performed multiplexed detection of three model proteins in HeLa cells.
Profiled β-amyloid and PHF-tau in formalin-fixed paraffin-embedded brain tissue.
Observed a correlated increase in β-amyloid and PHF-tau in Alzheimer's disease cohort.

Conclusions:

The developed multiplexed in-cell immunoassay is analytically powerful, simple, and robust.
This methodology enables insightful same-sample protein profiling.
Expected to be broadly adopted in biomedical research and clinical diagnostics.