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Related Concept Videos

Overview of Cell-Matrix Interactions01:24

Overview of Cell-Matrix Interactions

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The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...
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Cell Adhesion Molecules - Types and Functions01:20

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Cell adhesion molecules (CAMs) are pivotal to multicellularity and the coordinated functioning of tissues and organ systems. They enable physical interactions between cells and provide mechanical strength to tissues. They also function as receptors for signal transmission across the plasma membrane. The CAMs are broadly classified into four families - integrins, cadherins, selectins, and immunoglobulin-like CAMs (IgCAMs).
CAM Families
The Integrin family of proteins is primarily  involved...
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Cell Adhesion Molecules - Types and Functions01:20

Cell Adhesion Molecules - Types and Functions

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Immunoglobulin-like Cell Adhesion Molecules01:31

Immunoglobulin-like Cell Adhesion Molecules

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Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
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Anchoring Junctions01:03

Anchoring Junctions

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Anchoring junctions are multiprotein complexes that help cells connect to other cells and the extracellular matrix. Anchoring junctions are present on the lateral and basal surfaces of cells, providing strong and flexible connections. Focal adhesions are often formed due to cell interactions with the ECM substrata, which initiate signal transduction via kinase cascades and other mechanisms. Together, they provide stability and tissue integrity. There are three types of anchoring junctions:...
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Laminins are the Adhesive Proteins of Basal Lamina00:55

Laminins are the Adhesive Proteins of Basal Lamina

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Laminins are heterotrimeric proteins with high molecular mass found in the extracellular matrix. Each laminin molecule is composed of three chains, viz. alpha, beta, and gamma, coded by five, four, and three paralogous genes, respectively. Laminins are categories based on the compositions of the three chains.
In humans, the five forms of alpha chains are LAMA 1, LAMA 2, LAMA 3, LAMA 4, and LAMA 5. The four forms of beta chains are LAMB 1, LAMB 2, LAMB 3, and LAMB 4. The three forms of gamma...
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Related Experiment Video

Updated: Apr 4, 2026

Fabrication of a Biomimetic Nano-Matrix with Janus Base Nanotubes and Fibronectin for Stem Cell Adhesion
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Biotin-Avidin Based Universal Cell-Matrix Interaction for Promoting Three-Dimensional Cell Adhesion.

Xiao-Qiu Dou1, Jia Zhang1, Chuanliang Feng1

  • 1State Key Lab of Metal Matrix Composites, School of Materials Science and Engineering, Shanghai Jiaotong University , 800 Dongchuan Road, Shanghai, 200240, People's Republic of China.

ACS Applied Materials & Interfaces
|September 3, 2015
PubMed
Summary
This summary is machine-generated.

A novel biotin-avidin system enhances cell adhesion in 3D extracellular matrix (ECM), preventing cell death (anoikis). This universal method improves cell-matrix interactions for various cell types in biomaterials.

Keywords:
3D hydrogelbiotin−avidin interactioncell adhesionself-assemblysupramolecular gelator

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Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Tissue Engineering

Background:

  • Cell adhesion in three-dimensional (3D) extracellular matrix (ECM) is critical for cell survival, preventing anoikis.
  • Developing universal methods for promoting cell-matrix interactions is essential for fundamental cell biology and regenerative medicine.

Purpose of the Study:

  • To develop a universal cell-matrix interaction strategy using the biotin-avidin system to enhance cell adhesion in 3D ECM.
  • To create biotinylated nanofibrous hydrogels for improved cell integration and survival.

Main Methods:

  • Constructed biotinylated nanofibrous hydrogels via coassembly of non-biotinylated and biotinylated supramolecular gelators (1,4-benzyldicarboxamide based).
  • Modified cells with avidin (AV-cells) through biotinylation and subsequent avidin interaction.
  • Encapsulated AV-cells within the hydrogels and quantified cell adhesion.

Main Results:

  • The biotin-avidin interaction significantly increased cell adhesion by tens of percent with minimal addition of biotinylated gelators.
  • Reverse transcription polymerase chain reaction (RT-PCR) confirmed that AV-cells proliferated without altered gene expression or denaturation.
  • The avidin-biotin system demonstrated broader universality compared to RGD-cell interactions for promoting cell adhesion.

Conclusions:

  • The developed biotin-avidin based system provides a universal and effective method for promoting cell adhesion in 3D ECM.
  • This approach is highly feasible for enhancing cell integration and survival across diverse cell types within biomaterial scaffolds.
  • The strategy offers a promising tool for advancing cell biology research and tissue engineering applications.