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DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

DNA Isolation

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.

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Updated: Apr 4, 2026

Nuclei Isolation from Whole Tissue using a Detergent and Enzyme-Free Method

Nuclei Isolation from Whole Tissue using a Detergent and Enzyme-Free Method

Published on: June 24, 2020

Preparing Cellular DNA from Nuclei or Whole Cells.

Timothy W Nilsen

Cold Spring Harbor Protocols

|September 3, 2015

Summary

This summary is machine-generated.

This protocol details a method for isolating cellular DNA from nuclei, yielding material suitable for molecular analyses like polymerase chain reactions and Southern blots. The procedure ensures DNA stability for long-term storage and genetic investigations.

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Last Updated: Apr 4, 2026

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Preparation of Cytoplasmic and Nuclear Long RNAs from Primary and Cultured Cells

Preparation of Cytoplasmic and Nuclear Long RNAs from Primary and Cultured Cells

Published on: April 7, 2023

Area of Science:

Molecular Biology
Genetics

Background:

Cellular DNA is a critical resource for various molecular biology applications.
Maintaining DNA integrity is essential for accurate genetic analysis.

Purpose of the Study:

To describe a protocol for preparing high-quality cellular DNA from isolated nuclei.
To ensure DNA suitability for downstream applications such as PCR and Southern blotting.

Main Methods:

Isolation of cellular DNA from nuclei, excluding cytoplasmic components.
Removal of cytoplasmic extract to purify DNA.

Main Results:

The prepared DNA is suitable for polymerase chain reactions (PCR).
The DNA can be used for Southern blots to analyze plasmid integration and copy number in cell lines.
Potential for RNA contamination exists, affecting exact DNA quantitation.

Conclusions:

This method provides a reliable way to obtain stable cellular DNA for genetic studies.
The protocol is adaptable for whole-cell DNA extraction, though with increased RNA contamination.