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Generation of a Mouse Spontaneous Autoimmune Thyroiditis Model
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Generation of Tissue Sections for Screening Thy1 Mouse Lines.

Petar Marinković, Leanne Godinho, Thomas Misgeld

    Cold Spring Harbor Protocols
    |September 3, 2015
    PubMed
    Summary
    This summary is machine-generated.

    Researchers developed a new protocol to screen Thy1-XFP transgenic mice. This method uses histological characterization to detect Thy1-driven expression in young mice, aiding genetic research.

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    Area of Science:

    • Neuroscience
    • Genetics
    • Molecular Biology

    Background:

    • Thy1-XFP transgenic mice are valuable tools for studying gene expression.
    • Generating and screening these mice can be challenging.
    • Advances in pronuclear injection technology facilitate the creation of new transgenic lines.

    Purpose of the Study:

    • To provide a standardized protocol for screening Thy1-XFP transgenic mouse founder progeny.
    • To enable efficient detection of Thy1-driven fluorescent protein expression.
    • To support the use of Thy1-XFP mice in various research applications.

    Main Methods:

    • Crossing Thy1 transgenic founders with commercially obtained inbred mice.
    • Performing transcardial perfusion on 3-week-old F1 progeny.
    • Generating cryosections and performing histological characterization.
    • Detecting Thy1-driven expression using fluorescent protein markers.

    Main Results:

    • A reliable method for identifying Thy1-XFP transgenic founder mice was established.
    • Histological characterization effectively confirmed Thy1-driven expression.
    • The protocol allows for timely screening of young F1 mice.

    Conclusions:

    • This protocol simplifies the screening process for Thy1-XFP transgenic mice.
    • It facilitates the generation and utilization of these valuable research models.
    • The method supports advancements in neuroscience and genetic research.