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Imaging Acute Neuromuscular Explants from Thy1 Mouse Lines.

Petar Marinković, Leanne Godinho, Thomas Misgeld

    Cold Spring Harbor Protocols
    |September 3, 2015
    PubMed
    Summary
    This summary is machine-generated.

    Generating new Thy1-XFP transgenic mice is now accessible for smaller labs. This protocol details screening offspring using fluorescence microscopy on neuromuscular explants from young mice.

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    Area of Science:

    • Neuroscience
    • Genetics
    • Molecular Biology

    Background:

    • Transgenic mouse models are crucial for studying gene function.
    • Thy1 is a gene commonly used for neuronal expression systems.
    • Availability of core facilities has increased accessibility for generating transgenic animals.

    Purpose of the Study:

    • To provide a protocol for screening Thy1-XFP transgenic mouse offspring.
    • To enable researchers, including those in smaller labs, to generate and screen these valuable models.

    Main Methods:

    • Crossing Thy1 transgenic founder mice with commercially available inbred mice.
    • Obtaining acute neuromuscular explants from 3-week-old F1 offspring.
    • Utilizing fluorescence microscopy to detect Thy1-driven XFP expression.

    Main Results:

    • The protocol allows for the detection of Thy1-driven fluorescent protein expression in F1 offspring.
    • Successful screening identifies transgenic animals for further study.

    Conclusions:

    • This protocol facilitates the generation and screening of Thy1-XFP transgenic mice.
    • Increased accessibility of transgenic technologies empowers smaller research laboratories.