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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: Apr 4, 2026

Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow

Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow

Published on: November 4, 2016

Acoustofluidic Fluorescence Activated Cell Sorter.

Ahmad Ahsan Nawaz^1,2, Yuchao Chen¹, Nitesh Nama¹

¹Department of Engineering Science and Mechanics, The Pennsylvania State University , University Park, Pennsylvania 16802, United States.

Analytical Chemistry

|September 3, 2015

Summary

This summary is machine-generated.

This study introduces an acoustofluidic fluorescence activated cell sorting (FACS) device for high-throughput cell isolation. The novel chip integrates cell focusing and sorting, achieving high purity and cell viability for biological applications.

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Last Updated: Apr 4, 2026

Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow

Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow

Published on: November 4, 2016

Purification of Specific Cell Population by Fluorescence Activated Cell Sorting FACS

Purification of Specific Cell Population by Fluorescence Activated Cell Sorting FACS

Published on: July 10, 2010

Assembly and Operation of an Acoustofluidic Device for Enhanced Delivery of Molecular Compounds to Cells

Assembly and Operation of an Acoustofluidic Device for Enhanced Delivery of Molecular Compounds to Cells

Published on: January 21, 2021

Area of Science:

Biotechnology
Microfluidics
Cell Biology

Background:

Selective cell isolation is critical for biological research and clinical diagnostics.
Existing cell sorting methods face limitations in throughput, resolution, or complexity.

Purpose of the Study:

To develop an integrated acoustofluidic fluorescence activated cell sorting (FACS) device.
To achieve on-demand, high-throughput, and high-resolution cell detection and sorting on a single chip.

Main Methods:

Utilized microfluidic drifting for precise 3D cell focusing.
Employed standing surface acoustic waves (SSAW) for triggered, on-demand cell sorting.
Integrated laser interrogation and acoustic sorting on a single microfluidic chip.

Main Results:

Demonstrated continuous isolation of fluorescently labeled HeLa cells with high purity (92.3 ± 3.39%).
Achieved a high throughput of approximately 1200 events/s.
Maintained high post-sort cell viability (99.18%), indicating biocompatibility.

Conclusions:

The integrated acoustofluidic FACS device enables efficient, high-purity cell sorting.
The technology offers a promising platform for developing portable, user-friendly FACS instruments.
This device has significant potential for diverse biological studies and clinical applications.