Octadecyl ferulate behavior in 1,2-Dioleoylphosphocholine liposomes
Kervin O Evans1, David L Compton1, Nathan A Whitman1
1Renewable Products Technology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, United States Department of Agriculture, 1815 N. University Street, Peoria, IL 61604, USA.
Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|September 3, 2015
Summary
Octadecyl ferulate enhances liposome stability and antioxidant capacity. This lipid derivative forms domains in bilayers and increases phospholipid transition temperatures, showing potential in drug delivery applications.
Area of Science:
- Biochemistry
- Materials Science
- Physical Chemistry
Background:
- Octadecyl ferulate is a derivative of ferulic acid, a natural antioxidant.
- Liposomes are widely used as drug delivery vehicles.
- Understanding lipid-bilayer interactions is crucial for optimizing liposome formulations.
Purpose of the Study:
- To synthesize and characterize octadecyl ferulate.
- To investigate the effects of octadecyl ferulate on lipid bilayer properties.
- To evaluate the impact of octadecyl ferulate on liposome stability and antioxidant function.
Main Methods:
- Solid acid catalysis for synthesis.
- Supercritical Fluid Chromatography for monitoring.
- Differential Scanning Calorimetry (DSC) for phase transitions.
- Atomic Force Microscopy (AFM) for domain imaging.
- Fluorescence spectroscopy for liposome leakage and antioxidant capacity.
- Molecular modeling for interaction analysis.
Main Results:
- Octadecyl ferulate synthesized with 42% yield.
- DSC showed phase transitions at 67°C (melting) and 39°C (solidification).
- AFM revealed domain formation in lipid bilayers with 5-mol% octadecyl ferulate.
- Increased transition temperature of phospholipids observed.
- Enhanced liposome stability against leakage and maintained antioxidant capacity.
- Molecular modeling indicated antioxidant activity influenced by bilayer interactions.
Conclusions:
- Octadecyl ferulate can be synthesized and characterized.
- It influences lipid bilayer structure and phase behavior.
- Octadecyl ferulate improves liposome stability and retains antioxidant properties.
- Potential applications in enhancing liposomal drug delivery systems.
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