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Protein Engineering by Yeast Surface Display
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A switchable yeast display/secretion system.

James A Van Deventer1, Ryan L Kelly2, Saravanan Rajan3

  • 1Koch Institute for Integrative Cancer Research Department of Chemical Engineering James.Van_Deventer@tufts.edu sachdev.sidhu@utoronto.ca.

Protein Engineering, Design & Selection : PEDS
|September 4, 2015
PubMed
Summary
This summary is machine-generated.

This study introduces a yeast-based switchable display/secretion system for efficient antibody discovery. The novel method allows direct production of soluble antibody-like reagents, streamlining screening and characterization processes.

Keywords:
amber suppressionantibody screeningphage displaysynthetic antibody libraryyeast display

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Immunology

Background:

  • Traditional antibody discovery methods like immunization are time-consuming and require multiple steps.
  • Yeast and phage display technologies offer alternatives but necessitate converting displayed proteins to soluble forms for analysis.
  • A gap exists in seamlessly linking display-based screening with the production of soluble binding proteins.

Purpose of the Study:

  • To develop a yeast-based switchable display/secretion system for direct production of soluble antibody-like reagents.
  • To enable immediate generation of soluble reagents post-screening, simplifying downstream characterization.
  • To provide a cloning-free approach for generating affinity reagents.

Main Methods:

  • Utilized amber suppression technology to create a switchable yeast display and secretion system.
  • Performed model selections within the switchable format to assess efficiency.
  • Screened libraries in the switchable format to identify affinity reagents.

Main Results:

  • The switchable display/secretion system demonstrated efficient model selections.
  • Library screening yielded renewable sources of affinity reagents.
  • Candidate reagents exhibited high binding affinities in the nanomolar range.

Conclusions:

  • The developed system seamlessly integrates display-based screening with the production and evaluation of soluble binding proteins.
  • Switchable display/secretion libraries offer an accessible, cloning-free method for generating valuable affinity reagents.
  • This approach accelerates the discovery and development of antibody-like reagents for various applications.