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Related Concept Videos

MicroRNAs01:22

MicroRNAs

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
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Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR
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MicroRNA profiling in migraine without aura: pilot study.

Emmanuele Tafuri1,2, Donato Santovito3,4, Velia de Nardis3

  • 1a Emergency Department, "G. d'Annunzio" University of Chieti , Chieti , Italy.

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|September 4, 2015
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Summary

A specific profile of circulating microRNAs (miRNAs) is linked to migraine without aura. These miRNAs are also implicated in cardiovascular diseases, suggesting a potential diagnostic and pathophysiological connection.

Keywords:
Cardiovascular riskmicroRNAmigrainemigraine without aura

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Area of Science:

  • Biochemistry
  • Genetics
  • Neurology

Background:

  • MicroRNAs (miRNAs) are short, non-coding RNAs implicated in various diseases, including pain and conditions with increased cardiovascular (CV) risk.
  • The association between migraine without aura (MO) and CV risk is not well-established.
  • Identifying circulating biomarkers could aid in understanding MO pathophysiology and its CV implications.

Purpose of the Study:

  • To identify differentially expressed circulating miRNAs in patients with MO.
  • To explore the potential of miRNA profiles as diagnostic markers for MO.
  • To investigate the link between MO and CV risk through miRNA expression.

Main Methods:

  • Circulating miRNA expression profiling in 15 female MO patients and 13 healthy controls.
  • Validation of selected miRNAs (miR-22, miR-26a, miR-26b, miR-27b, miR-29b, let-7b, miR-181a, miR-221, miR-30b, miR-30e) using quantitative real-time polymerase chain reaction.
  • Development of a logistic regression model to assess diagnostic accuracy.

Main Results:

  • Four miRNAs were differentially expressed in MO patients compared to controls: miR-27b (up-regulated), miR-181a, let-7b, and miR-22 (down-regulated).
  • Down-regulation of miR-22 and let-7b was confirmed in circulating blood monocytes.
  • A miRNA expression profile model demonstrated high accuracy in identifying MO (AUC: 0.956, P < 0.001).

Conclusions:

  • A distinct circulating miRNA profile is associated with MO.
  • The identified miRNAs are also modulated in atherosclerosis and stroke, suggesting a potential link to CV risk.
  • This study provides a foundation for further research into MO diagnosis, pathophysiology, and its cardiovascular implications.