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Updated: Apr 4, 2026
Two-layered Membrane Sandwich Method for Laodelphax striatellus Saliva Collection
Published on: August 27, 2021
A Deep Insight Into the Sialotranscriptome of the Chagas Disease Vector, Panstrongylus megistus (Hemiptera:
José M C Ribeiro1, Alexandra Schwarz2, Ivo M B Francischetti3
1National Institute of Allergy and Infectious Diseases, Laboratory of Malaria and Vector Research, 12735 Twinbrook Parkway, MD 20852. jribeiro@niaid.nih.gov.
Abstract:
Saliva of blood-sucking arthropods contains a complex cocktail of pharmacologically active compounds that assists feeding by counteracting their hosts' hemostatic and inflammatory reactions. Panstrongylus megistus (Burmeister) is an important vector of Chagas disease in South America, but despite its importance there is only one salivary protein sequence publicly deposited in GenBank. In the present work, we used Illumina technology to disclose and publicly deposit 3,703 coding sequences obtained from the assembly of >70 million reads. These sequences should assist proteomic experiments aimed at identifying pharmacologically active proteins and immunological markers of vector exposure. A supplemental file of the transcriptome and deducted protein sequences can be obtained from http://exon.niaid.nih.gov/transcriptome/P_megistus/Pmeg-web.xlsx.
