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TRIP-Br1 oncoprotein inhibits autophagy, apoptosis, and necroptosis under nutrient/serum-deprived condition

Samil Jung1, Chengping Li1, Jingjing Duan1

  • 1Department of Life Systems, Sookmyung Women's University, Seoul, 140-742, South Korea.

Oncotarget
|September 4, 2015
PubMed

Insights

The TRIP-Br1 oncoprotein promotes cancer cell survival by blocking cell death pathways like apoptosis and necroptosis, especially under nutrient-deprived conditions. Its elevated expression in breast cancer cells helps them resist serum starvation.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • TRIP-Br1 is an oncogenic protein with diverse cellular functions.
  • Cancer cells require mechanisms to survive stressful conditions like nutrient deprivation.

Purpose of the Study:

  • To investigate the role of TRIP-Br1 in cancer cell survival under nutrient/serum starvation.
  • To elucidate the molecular mechanisms by which TRIP-Br1 promotes cancer cell resistance.

Main Methods:

  • Culturing breast cancer cell lines (MCF7, MDA-MB-231, etc.) under nutrient-depleted conditions (serum starvation, glucose/amino acid deprivation).
  • Utilizing TRIP-Br1 silencing siRNA to assess its impact on cell death.
  • Measuring XIAP protein levels and cellular ROS production.
  • Analyzing the involvement of the PI3K/AKT signaling pathway.

Main Results:

  • TRIP-Br1 expression significantly increased in cancer cells under nutrient/serum starvation but not in normal cells.
  • TRIP-Br1 silencing accelerated apoptosis and necroptosis in breast cancer cells.
  • TRIP-Br1 confers resistance to serum starvation by stabilizing XIAP and inhibiting ROS production.
  • Serum starvation-induced TRIP-Br1 increase involves partial blockage of the PI3K/AKT pathway.

Conclusions:

  • TRIP-Br1 acts as an oncoprotein by inhibiting autophagy, apoptosis, and necroptosis, enhancing cancer cell survival during nutrient/serum starvation.
  • TRIP-Br1's protective role involves XIAP stabilization and ROS inhibition, potentially mediated by the PI3K/AKT pathway.
Keywords:
TRIP-Br1apoptosisautophagynecroptosisnutrient/serum starvation

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