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Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

Fragment ion patchwork quantification for measuring site-specific acetylation degrees.

Rasha ElBashir, Jens T Vanselow, Amelie Kraus

¹Department of Cell & Developmental Biology, Biocenter University of Wuerzburg , Am Hubland, 97074 Wuerzburg, Germany.

Analytical Chemistry

|September 4, 2015

Summary

This summary is machine-generated.

We developed fragment ion patchwork quantification, a mass spectrometry method for precise site-specific acetylation measurement. This technique accurately quantifies acetylation on all lysine residues in core histones from Trypanosoma brucei.

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Area of Science:

Biochemistry
Proteomics
Mass Spectrometry

Background:

Histone acetylation plays a crucial role in gene regulation.
Accurate quantification of site-specific acetylation is essential for understanding its functional roles.
Existing methods may have limitations in comprehensively analyzing histone acetylation patterns.

Purpose of the Study:

To introduce and validate a novel mass spectrometry-based approach for highly accurate site-specific acetylation quantification.
To demonstrate the applicability of this method for analyzing all lysine residues in core histones.
To showcase its utility in identifying histone acetyltransferase substrates.

Main Methods:

Fragment ion patchwork quantification combines (13)C1-acetyl derivatization at the protein level.
Utilizes proteolysis by low-specificity proteases.
Quantification is performed at the fragment ion level, analyzing isotope patterns of acetylated b and y ions.

Main Results:

Successfully determined site-specific acetylation degrees for all lysine residues in core histones of Trypanosoma brucei.
Demonstrated the method's capability to identify substrate sites for histone acetyltransferases.
Achieved high accuracy in quantifying acetylation degrees.

Conclusions:

Fragment ion patchwork quantification is a powerful new tool for precise analysis of site-specific protein acetylation.
This method enables comprehensive profiling of histone acetylation.
It facilitates the discovery of novel acetylation sites and enzyme substrates.